Abstract | OBJECTIVE: METHODS: Sixty SD male rats were randomly divided into normal control, AMI model, Neiguan (PC 6), Lieque (LU 7) and non- acupoint groups. The AMI model was established by intravenous injection of Isoprenaline (85 mg/kg) once daily for 2 days. Myocardial Nav 1.5 and Nav β 1, β 2, β 3, β 4 protein expression levels were detected by Western blot. RESULTS: In comparison with the control group, myocardial Nav 1.5, β 1, β 2, β 3 and β 4 protein expression levels were significantly down-regulated in the AMI model group (P<0.01). After EA stimulation, compared with the model group, the expression levels of Nav 1.5, Nav β 1, β 2, β 3 and β 4 protein expression levels were significantly up-regulated in the Neiguang (PC 6) and Lieque (LU 7) groups (P<0.01) rather than in the non- acupoint group (P>0.05). There was no statistical significance between the Neiguan (PC 6) and Lieque (LU 7) groups in β 4 protein expression level (P>0.05). CONCLUSION: EA stimulation of both PC 6 and LU 7 can significantly reverse AMI-induced down-regulation of myocardial Nav 1.5, β 1, β 2, β 3 and β 4 protein expression levels in AMI rats, which might contribute to its function in improving AMI by reducing calcium overload.
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Authors | Di Bian, Yi-guo Chen, Yue-jiao Sui, Hui Tian, Yu-li Liu, Rui Cao, Cheng-lin Li, Bao-yan Li |
Journal | Zhen ci yan jiu = Acupuncture research
(Zhen Ci Yan Jiu)
Vol. 40
Issue 1
Pg. 45-9
(Feb 2015)
ISSN: 1000-0607 [Print] China |
PMID | 25845220
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Protein Subunits
- Voltage-Gated Sodium Channels
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Topics |
- Acupuncture Points
- Animals
- Electroacupuncture
- Humans
- Male
- Myocardial Ischemia
(genetics, metabolism, therapy)
- Myocardium
(metabolism)
- Protein Subunits
(genetics, metabolism)
- Rats
- Rats, Sprague-Dawley
- Voltage-Gated Sodium Channels
(genetics, metabolism)
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