Abstract | PURPOSE: To evaluate the cytotoxicity of components released from different types of luting cements to two cell lines obtained from pulp tissue. METHODS: Three types of luting cements were evaluated, distributed into the following groups: G1--negative control (no treatment); G2--resin-modified glass-ionomer cement ( Rely X Luting 2); G3--self-adhesive resin cement ( Rely X U200); and G4--conventional resin cement ( Rely X ARC). Standardized cylindrical specimens (14 mm diameter and 1 mm thick) prepared with the dental materials were immersed in culture medium (DMEM) for 24 hours to obtain the extracts (DMEM + components released from the cements). Then, the extracts were applied to cultured odontoblast-like MDPC-23 cells or human dental pulp cells (HDPCs). Finally, cell viability (MTT assay), cell death ( Annexin/PI) (Kruskal-Wallis/Mann-Whitney; α = 5%) and cell morphology (SEM) were assessed. Cements' components in contact with cells (SEM/EDS) and pH of the extracts were also evaluated. RESULTS: The resin-modified glass-ionomer cement (G2) caused the most intense toxic effect to the two cell lines; the cell viability reduction was around 95.8% and 89.4% for MDPC-23 cells and HDPCs, respectively, which was statistically significantly different compared with that of the negative control group (G1). Also, a high quantity of particles leached from this ionomeric cement was found on the cells, which showed intense morphological alterations. In the G2 group, 100% necrosis was observed for both cell lines, and an acidic pH was detected on the extract. Conversely, Rely X U200 (G3) and Rely X ARC (G4), which presented low solubility and no alteration in pH, caused only slight cytotoxicity to the cultured cells.
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Authors | Elaine Cristina Voltolini Pontes, Diana Gabriela Soares, Josimeri Hebling, Carlos Alberto De Souza Costa |
Journal | American journal of dentistry
(Am J Dent)
Vol. 27
Issue 5
Pg. 237-44
(Oct 2014)
ISSN: 0894-8275 [Print] United States |
PMID | 25842455
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Cell Line
- Dental Cements
(toxicity)
- Dental Pulp
(cytology, drug effects)
- Microscopy, Electron, Scanning
- Rats
- Spectrometry, X-Ray Emission
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