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Autoantigen complementarity' is a theory proposing that the initiator of an autoimmune response is not necessarily the
autoantigen or its molecular mimic, but may instead be a
peptide that is 'antisense/complementary' to the
autoantigen. We investigated whether such complementary
proteins play a role in the immunopathogenesis of autoimmune
glomerulonephritis. Experimental autoimmune
glomerulonephritis, a model of anti-glomerular basement membrane (GBM) disease, can be induced in Wistar Kyoto (WKY) rats by immunization with the α3 chain of
type IV collagen. In this study, WKY rats were immunized with a complementary α3
peptide (c-α3-Gly) comprised of
amino acids that '
complement' the well characterized
epitope on α3(IV)NC1, pCol(24-38). Within 8 weeks post-immunization, these animals developed cresentic
glomerulonephritis, similar to pCol(24-38)-immunized rats, while animals immunized with scrambled
peptide were normal.
Anti-idiotypic antibodies to
epitopes from c-α3-Gly-immunized animals were shown to be specific for α3 protein, binding in a region containing sense pCol(24-38) sequence. Interestingly, anti-complementary α3
antibodies were identified in sera from patients with
anti-GBM disease, suggesting a role for '
autoantigen complementarity' in immunopathogenesis of the human disease. This work supports the idea that autoimmune
glomerulonephritis can be initiated through an immune response against a
peptide that is anti-sense or complementary to the
autoantigen. The implications of this discovery may be far reaching, and other
autoimmune diseases could be due to responses to these once unsuspected 'complementary'
antigens.