Aromatase inhibitor (anastrozole) affects growth of endometrioma cells in culture.

Abstract

OBJECTIVE:

To study the effects of aromatase inhibitor (anastrozole) on the growth and estradiol secretion of endometrioma cells in culture.

DESIGN:

Endometrioma cells are grown in vitro until maximum growth before used in this study. This was done in the research laboratory for tissue culture, in an academic hospital.

INTERVENTIONS:

Testosterone at a concentration of 10 μg/mL was added as a substrate for the intracellular aromatase. In addition, aromatase inhibitor was added at a concentration of 200 and 300 μg/mL. The effect on cell growth and estradiol secretion is evaluated using Student's t-test.

RESULTS:

The use of testosterone increased estradiol secretion by endometrioma cells in culture. The use of aromatase inhibitor significantly inhibited the growth of endometrioma cells, and estradiol secretion.

CONCLUSION:

Aromatase inhibitor (anastrozole) may be an effective treatment for endometriosis due to inhibition of cellular aromatase.

• Authors: Shawky Z A Badawy, Shereene Brown, Lydia Kaufman, Martha A Wojtowycz
• Journal: European journal of obstetrics, gynecology, and reproductive biology (Eur J Obstet Gynecol Reprod Biol) Vol. 188 Pg. 45-50 (May 2015) ISSN: 1872-7654 [Electronic] Ireland
• PMID: 25839436 (Publication Type: Journal Article)
• Copyright: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Aromatase Inhibitors
• Nitriles
• Triazoles
• Anastrozole
• Testosterone
• Estradiol

Topics

• Anastrozole
• Aromatase Inhibitors (pharmacology)
• Cell Proliferation (drug effects)
• Cells, Cultured
• Endometriosis (metabolism, pathology)
• Estradiol (metabolism)
• Female
• Humans
• Nitriles (pharmacology)
• Testosterone (pharmacology)
• Triazoles (pharmacology)