High-Affinity H7 Head and Stalk Domain-Specific Antibody Responses to an Inactivated Influenza H7N7 Vaccine After Priming With Live Attenuated Influenza Vaccine.

Abstract

Recent studies have shown that live attenuated influenza vaccines (LAIVs) expressing avian influenza virus hemagglutinins (HAs) prime for strong protective antibody responses to an inactivated influenza vaccine (IIV) containing the HA. To better understand this priming effect, we compared H7 HA head and stalk domain-specific B-cell responses in H7N7 LAIV-primed subjects and non-H7-primed controls after a single dose of H7N7 IIV. As previously reported, H7N7 LAIV-primed subjects but not control subjects generated strong hemagglutination-inhibiting and neutralizing antibody responses to the H7N7 IIV. Here, we found that the quantity, epitope diversity, and affinity of H7 head-specific antibodies increased rapidly in only H7N7 LAIV-primed subjects after receipt of the IIV. However, all cohorts generated a vigorous, high-affinity, stalk-specific antibody response. Consistent increases in circulating memory B-cell frequencies after receipt of the IIV reflected the specificity of high-affinity antibody production. Our findings emphasize the value of LAIVs as a vehicle for prepandemic vaccination.

• Authors: Jessica L Halliley, Surender Khurana, Florian Krammer, Theresa Fitzgerald, Elizabeth M Coyle, Ka Yan Chung, Steven F Baker, Hongmei Yang, Luis Martínez-Sobrido, John J Treanor, Kanta Subbarao, Hana Golding, David J Topham, Mark Y Sangster
• Journal: The Journal of infectious diseases (J Infect Dis) Vol. 212 Issue 8 Pg. 1270-8 (Oct 15 2015) ISSN: 1537-6613 [Electronic] United States
• PMID: 25838266 (Publication Type: Clinical Trial, Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: © The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: [email protected].

Chemical References

• Antibodies, Neutralizing
• Antibodies, Viral
• Influenza Vaccines
• Vaccines, Attenuated
• Vaccines, Inactivated

Topics

• Antibodies, Neutralizing (blood)
• Antibodies, Viral (blood)
• B-Lymphocytes (immunology)
• Cohort Studies
• Hemagglutination Inhibition Tests
• Humans
• Influenza A Virus, H7N7 Subtype (immunology)
• Influenza Vaccines (immunology)
• Influenza, Human (immunology, prevention & control, virology)
• Neutralization Tests
• Vaccines, Attenuated (immunology)
• Vaccines, Inactivated (immunology)