cCMP and cUMP occur in vivo.

Abstract

Mammalian cells contain the cyclic pyrimidine nucleotides cCMP and cUMP. It is unknown whether these tentative new second messenger molecules occur in vivo. We used high performance liquid chromatography quadrupole tandem mass spectrometry to quantitate nucleoside 3',5'-cyclic monophosphates. cCMP was detected in all organs studied, most notably pancreas, spleen and the female reproductive system. cUMP was not detected in organs, probably due to the intrinsically low sensitivity of mass spectrometry to detect this molecule and organ matrix effects. Intratracheal infection of mice with recombinant Pseudomonas aeruginosa harboring the nucleotidyl cyclase toxin ExoY massively increased cUMP in lung. The identity of cCMP and cUMP in organs was confirmed by high performance liquid chromatography quadrupole time of flight mass spectrometry. cUMP also appeared in serum, urine and faeces following infection. Taken together, this report unequivocally shows for the first time that cCMP and cUMP occur in vivo.

• Authors: Heike Bähre, Christina Hartwig, Antje Munder, Sabine Wolter, Tane Stelzer, Bastian Schirmer, Ulrike Beckert, Dara W Frank, Burkhard Tümmler, Volkhard Kaever, Roland Seifert
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 460 Issue 4 Pg. 909-14 (May 15 2015) ISSN: 1090-2104 [Electronic] United States
• PMID: 25838203 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Nucleotides, Cyclic
• Cyclic CMP
• cyclic 3',5'-uridine monophosphate
• Uridine Monophosphate

Topics

• Animals
• Chromatography, High Pressure Liquid
• Cyclic CMP (metabolism)
• Female
• Mice
• Mice, Inbred C57BL
• Nucleotides, Cyclic (metabolism)
• Real-Time Polymerase Chain Reaction
• Tandem Mass Spectrometry
• Uridine Monophosphate (metabolism)