Abstract | BACKGROUND: OBJECTIVE: We evaluated the potential anti-inflammatory activity of EPS by measuring its antioxidant potential in skin. METHODS: RESULTS: OX-induced contact dermatitis was suppressed markedly in mice that were treated with an ointment containing 5% EPS as evidenced by a decrease in the extent of scaling and thickening (p<0.05) and supported by a histological study. COX-2 ( messenger RNA [ mRNA] and protein) and NGF ( mRNA) levels, which were upregulated in the skin of OX-treated mice, were suppressed markedly in the skin of OX+EPS-treated mice. Consistent with this, compound 48/80-induced expression of COX-2 ( mRNA and protein) and NGF ( mRNA) in HaCaT cells were suppressed by EPS treatment in a dose-dependent manner. As an inhibitor of NF-κB, IκB protein levels were dose-dependently upregulated by EPS. Fluorescence-activated cell sorting (FACS) analysis revealed that EPS scavenged compound 48/80-induced reactive oxygen species (ROS) in HaCaT cells. CONCLUSION:
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Authors | Da-In Choi, Jee-Young Choi, Young Jee Kim, Jee-Bum Lee, Sun-Ouck Kim, Hyong-Taek Shin, Seung-Chul Lee |
Journal | Annals of dermatology
(Ann Dermatol)
Vol. 27
Issue 2
Pg. 142-51
(Apr 2015)
ISSN: 1013-9087 [Print] Korea (South) |
PMID | 25834352
(Publication Type: Journal Article)
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