Primary analysis and screening of microRNAs in gastric cancer side population cells.

Abstract

AIM:

To explore the microRNA (miRNA) profiles and to determine the key miRNAs within the side population (SP) cells of the gastric cancer cell line MKN-45.

METHODS:

We used fluorescence-activated cell sorting and Hoechst 33342 labeling to obtain SP cells from the human gastric carcinoma cell line MKN-45. The miRNA expression profiles of the SP and major population (MP) cells were examined using a miRNA gene chip, and key miRNAs were obtained according to aberrant expression and the miRNAs' possible targets as predicted by bioinformatics.

RESULTS:

Using a significance criterion of a 1.5-fold or greater difference in expression level, we observed an increase in the expression of 34 miRNAs and a decrease in the expression of 34 miRNAs when comparing SP to MP cells. Using quantitative real-time reverse transcription-polymerase chain reaction to test for differentially expressed miRNAs combined with bioinformatics results, we found that the downregulated miRNAs, such as hsa-miR-3175 and hsa-miR-203, and the upregulated miRNAs, including hsa-miR-130a, hsa-miR-324-5p, hsa-miR-34a, and hsa-miR-25-star, may be important in maintaining and regulating the characteristics of SP cells.

CONCLUSION:

There are key miRNAs expressed within the SP cells of the gastric cancer cell line MKN-45, and include hsa-miR-3175, hsa-miR-203, hsa-miR-130a, hsa-miR-324-5p, hsa-miR-34a, and hsa-miR-25-star.

• Authors: Hai-Hong Zhang, Guo-Li Gu, Xiang-Yang Zhang, Feng-Zhi Li, Li Ding, Qin Fan, Ran Wu, Wei Shi, Xin-Yan Wang, Lin Chen, Xue-Ming Wei, Xiao-Ying Yuan
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 21 Issue 12 Pg. 3519-26 (Mar 28 2015) ISSN: 2219-2840 [Electronic] United States
• PMID: 25834316 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MicroRNAs

Topics

• Adenocarcinoma (genetics, metabolism, pathology)
• Cell Line, Tumor
• Computational Biology
• Gene Expression Profiling (methods)
• Gene Expression Regulation, Neoplastic
• Humans
• MicroRNAs (genetics, metabolism)
• Oligonucleotide Array Sequence Analysis
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction
• Side-Population Cells (metabolism, pathology)
• Stomach Neoplasms (genetics, metabolism, pathology)