Abstract |
Decabrominated diphenyl ether (PBDE-209) is a persistent organic pollutant. Gestational exposure to PBDE-209 can accumulate in pregnant women and fetuses via the placenta and umbilical cord, affecting perinatal outcome. In this study, pregnant Sprague-Dawley (SD) rats were randomly divided into five groups and intragastrically administered peanut oil (vehicle) 1, 5 and 10mg/kg by body weight (b.w.) of PBDE-209, or nothing (control) from day 0 (G0) to day 21 (G21) gestation, respectively. Placental samples were collected on G21 by cesarean section. The mRNA and protein expressions of ET-1, eNOS and iNOS in the placenta were examined using qRT-PCR and Western blot, respectively. Total nitric oxide (NO) in the placenta was measured using a specific ELISA kit. Compared with the control and vehicle groups, the mRNA expression of ET-1 and iNOS in the placenta was gradually and significantly increased after exposure to increasing concentrations of PBDE-209 (P<0.05), while the mRNA level of eNOS in the placenta was gradually and significantly reduced after exposure to increasing concentrations of PBDE-209 (P<0.05). The expression trends of ET-1, eNOS and iNOS proteins were consistent with those of mRNA expression. Interestingly, the production of total NO was significantly increased after exposure to 5 and 10mg/kg b.w. PBDE-209 (P<0.05). Finally, the birth weight of the offspring rats was significantly reduced after maternal exposure to 5 and 10 mg/kg b.w. PBDE-209 compared with the control and vehicle groups (P<0.05). These results suggest that PBDE-209 exposure during pregnancy upregulates ET-1 and iNOS expression, but decreases eNOS expression in the placenta, as well as reduces the birth weight of offspring.
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Authors | Peili Du, Zhihua Li, Lili Du, Huili Zhang, Yanmei Zhou, Wen Sun, Xue Xiao, Yutian He, Bin Sun, Yanhong Yu, Dunjin Chen |
Journal | International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience
(Int J Dev Neurosci)
Vol. 43
Pg. 86-91
(Jun 2015)
ISSN: 1873-474X [Electronic] United States |
PMID | 25824818
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Ltd. All rights reserved. |
Chemical References |
- Endothelin-1
- Halogenated Diphenyl Ethers
- RNA, Messenger
- Nitric Oxide Synthase Type III
- decabromobiphenyl ether
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Topics |
- Analysis of Variance
- Animals
- Body Weight
(drug effects)
- Dose-Response Relationship, Drug
- Endothelin-1
(genetics, metabolism)
- Female
- Gene Expression Regulation, Developmental
(drug effects)
- Halogenated Diphenyl Ethers
(toxicity)
- Male
- Nitric Oxide Synthase Type III
(genetics, metabolism)
- Placenta
(drug effects, metabolism)
- Pregnancy
- Prenatal Exposure Delayed Effects
(chemically induced, physiopathology)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
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