[Calf blood hemodializate reduces neurotoxic effect of hydrogen peroxide on human neuroblastoma cells].

Abstract

OBJECTIVE:

Our aim was to study the effect of calf blood gemodializat on apoptosis and intracellular signaling pathways of neuroblastoma cells SK-N-SH human.

METHODS:

Apoptosis was recorded by fluorescent microscopy using Hoechst 33342. Necrosis cells was monitored by propidium iodide. The fluorescence of the cells was recorded on a fluorescence inverted microscope Keyence BZ8100 (Japan). Formation of reactive oxygen species (ROS) in the cells of SK-N-SH was determined using nitroblue tetrazolium by absorbance at 620 nm on a plate reader "Uniplan".

RESULTS:

When adding hydrogen peroxide to the background of the calf blood gemodializat been decreasing apoptosis of these cells with 43 to 17% relative to apoptosis in the presence of a hydrogen peroxide. Under these conditions, the calf blood gemodializat significantly reduced ROS formation in human neuroblastoma cells SK-N-SH by the action of hydrogen peroxide. In these cells, we investigated the influence of calf blood gemodializat on apoptosis and intracellular signaling pathway involving mitogen-activated protein kinase (p38MAPK), extracellular regulatory kinase (ERK), phosphatidylinositol 3-kinase (PI-3K) and-Jun-N-terminal kinase (JNK) using their selective inhibitors.

CONCLUSION:

It was shown that the mechanism of the protective effect of calf blood gemodializat against peroxide-induced apoptosis in SK-N-SH dominant role is played by p38 MAPK and PI-3K.

• Authors: M M Yurinskaya, M G Vinokurov, E I Astashkin, S V Grachev, N S Orekhova, A N Novikova, I N Sokolova
• Journal: Vestnik Rossiiskoi akademii meditsinskikh nauk (Vestn Ross Akad Med Nauk) Issue 9-10 Pg. 10-4 ( 2014) ISSN: 0869-6047 [Print] Russia (Federation)
• PMID: 25816637 (Publication Type: Journal Article)

Chemical References

• Enzyme Inhibitors
• Flavonoids
• Imidazoles
• Pyridines
• Reactive Oxygen Species
• Hydrogen Peroxide
• Phosphatidylinositol 3-Kinase
• p38 Mitogen-Activated Protein Kinases
• MAP Kinase Kinase 4
• SB 203580
• 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one

Topics

• Animals
• Apoptosis (drug effects)
• Cattle
• Cell Line, Tumor
• Enzyme Inhibitors (pharmacology)
• Flavonoids (pharmacology)
• Humans
• Hydrogen Peroxide (toxicity)
• Imidazoles (pharmacology)
• MAP Kinase Kinase 4 (antagonists & inhibitors, metabolism)
• Neuroblastoma (drug therapy, metabolism, pathology)
• Neurotoxicity Syndromes (pathology)
• Phosphatidylinositol 3-Kinase (metabolism)
• Pyridines (pharmacology)
• Reactive Oxygen Species (metabolism)
• Renal Dialysis
• Signal Transduction (drug effects)
• p38 Mitogen-Activated Protein Kinases (antagonists & inhibitors, metabolism)