Ophiopogonin-D suppresses MDA-MB-435 cell adhesion and invasion by inhibiting matrix metalloproteinase-9.

Abstract

Ophiopogonin-D is one of steroidal saponins isolated from the root of the Chinese medicinal plant Ophiopogon japonicas. It has been claimed to possess anti-inflammatory and anti-oxidant properties. The present study was the first to examine the anti-tumor metastasis properties of ophiopogonin-D. An MTT assay showed that ophiopogonin-D inhibited the proliferation of MDA-MB-435 melanoma cells, and decreased invasion was demonstrated using a Transwell invasion assay. Furthermore, adhesion of MDA-MB-435 cells to human umbilical vascular endothelial cells and to fibronectin was inhibited by ophiopogonin-D. Gelatin zymography and western blot analysis showed that ophiopogonin-D inhibited the expression and secretion of matrix metalloproteinase-9 (MMP-9), but not that of MMP-2. Inhibition of phosphorylation of p38 by ophiopogonin-D indicated its inhibition of the mitogen-activated protein kinase pathway. Overall, the results suggested that ophiopogonin-D may be considered as a candidate drug for treating or preventing tumor metastasis.

• Authors: Yuanyuan Zhang, Yuwei Han, Kefeng Zhai, Minhui Sun, Jihua Liu, Boyang Yu, Junping Kou
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 12 Issue 1 Pg. 1493-8 (Jul 2015) ISSN: 1791-3004 [Electronic] Greece
• PMID: 25816153 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Drugs, Chinese Herbal
• Isoflavones
• NF-kappa B
• ophiopogonone D
• Matrix Metalloproteinase 2
• MMP9 protein, human
• Matrix Metalloproteinase 9

Topics

• Antineoplastic Agents, Phytogenic (administration & dosage)
• Cell Adhesion (drug effects)
• Cell Line, Tumor
• Drugs, Chinese Herbal (administration & dosage, chemistry)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Isoflavones (administration & dosage)
• Matrix Metalloproteinase 2 (biosynthesis, genetics)
• Matrix Metalloproteinase 9 (biosynthesis, drug effects, genetics)
• Melanoma (drug therapy, genetics, pathology)
• NF-kappa B (metabolism)
• Neoplasm Invasiveness (genetics)
• Phosphorylation (drug effects)