Anti-inflammatory effects of Gualou Guizhi decoction in transient focal cerebral ischemic brains. [Corrected].

Abstract

The aim of the present study was to explore the neuroprotective effects of Gualou Guizhi decoction (GLGZD) in a rat model of middle cerebral artery occlusion (MCAO). Sprague-Dawley rats were divided into three groups: Sham (no MCAO), MCAO (MCAO with no GLGZD treatment) and GLGZD (MCAO with GLGZD treatment). Rats in the MCAO and GLGZD groups were subjected to permanent occlusion of the left middle cerebral artery. Neurological function and infarct volume were measured. Microglial activation and inflammatory cell accumulation were measured using immunohistochemistry. mRNA and protein expression of inflammatory mediators were examined using reverse transcription-quantitative polymerase chain reaction and an enzyme-linked immunosorbent assay. The expression of proteins associated with the nuclear factor κ-B (NF-κB) inflammation signaling pathway was analyzed using western blotting. The results of the present study suggested that infarct size was significantly reduced and neurological behavior function was improved in rats with MCAO treated with GLGZD compared with rats in the MCAO group. Amoeboid microglial expansion and inflammatory cell migration were observed in the infarcted areas of rats in the GLGZD group and were not identified in those of the MCAO group. Target mRNA and protein levels, and inflammatory cell infiltration were significantly reduced in the GLGZD group compared with the MCAO model group. Notably, GLGZD treatment induced neuroprotective effects, reducing inflammation and inhibiting NF-κB signaling compared with the MCAO group. Therefore, GLGZD may exhibit anti-inflammatory effects against ischemia-reperfusion brain injury and may be a therapeutic target for ischemic stroke.

• Authors: Hai Xia Hu, Ru Hui Lin, Xiao Qin Zhu, Zuan Fang Li, Li Dian Chen
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 12 Issue 1 Pg. 1321-7 (Jul 2015) ISSN: 1791-3004 [Electronic] Greece
• PMID: 25815521 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drugs, Chinese Herbal
• NF-kappa B
• Neuroprotective Agents
• guizhi decoction

Topics

• Animals
• Brain (drug effects, physiopathology)
• Disease Models, Animal
• Drugs, Chinese Herbal (administration & dosage)
• Gene Expression Regulation (drug effects)
• Humans
• Infarction, Middle Cerebral Artery
• Inflammation (drug therapy, genetics, physiopathology)
• Ischemic Attack, Transient (drug therapy, genetics, physiopathology)
• NF-kappa B (biosynthesis, genetics)
• Neuroprotective Agents (administration & dosage)
• Rats
• Reperfusion Injury (drug therapy, genetics, physiopathology)
• Signal Transduction
• Stroke (drug therapy, genetics, physiopathology)