Abstract |
A cytophotometrical study was made of Fast Green FCF binding by nuclear non- histone proteins of the rat superior ganglion neurons at pH 2.6 (1 day, 1 week, 1 and 5 months, and 2.5 years after birth, a. b.). An increased dye binding by fraction NHP, stable in 0.35 M NaCl, was seen during the terminal cytodifferentiation (between days 7 and 14 a. b.). The extraction of a loosely bound fraction of non- histone proteins caused a decrease in the transcription level evaluated by the Moore method, throughout the whole postnatal ontogenesis, and no effect of extraction was noticed in respect of the AS-staining of nuclear histones.
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Authors | A V Grigor'eva, V N Iarygin |
Journal | Tsitologiia
(Tsitologiia)
Vol. 27
Issue 2
Pg. 191-5
(Feb 1985)
ISSN: 0041-3771 [Print] Russia (Federation) |
Vernacular Title | Tsitologicheskiĭ analiz belkovogo komponenta iadernogo khromatina simpaticheskikh nevrotsitov krysy v postnatal'nom ontogeneze. II. Vozrastnye izmeneniia negistonovykh iadernykh belkov, vyiavliaemye prochnym zelenym, i matrichnaia aktivnost' do i posle ékstraktsii slabosviazannoĭ fraktsii. |
PMID | 2581342
(Publication Type: Comparative Study, English Abstract, Journal Article)
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Chemical References |
- Chromosomal Proteins, Non-Histone
- Histones
- Lissamine Green Dyes
- ammoniacal silver
- Silver
- Fast Green FCF
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Topics |
- Aging
- Animals
- Cell Differentiation
- Cell Nucleus
(metabolism, ultrastructure)
- Chromosomal Proteins, Non-Histone
(isolation & purification, metabolism)
- Ganglia, Sympathetic
(metabolism, ultrastructure)
- Histones
(metabolism)
- Lissamine Green Dyes
- Neurons
(metabolism, ultrastructure)
- Nissl Bodies
(metabolism, ultrastructure)
- Rats
- Silver
- Staining and Labeling
(methods)
- Transcription, Genetic
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