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[Cytological analysis of the protein component of the nuclear chromatin in rat sympathetic neurocytes in postnatal ontogeny. II. The age-related changes in the nonhistone chromosomal proteins detectable by fast green and the matrix activity before and after extraction of the weakly bound fraction].

Abstract
A cytophotometrical study was made of Fast Green FCF binding by nuclear non-histone proteins of the rat superior ganglion neurons at pH 2.6 (1 day, 1 week, 1 and 5 months, and 2.5 years after birth, a. b.). An increased dye binding by fraction NHP, stable in 0.35 M NaCl, was seen during the terminal cytodifferentiation (between days 7 and 14 a. b.). The extraction of a loosely bound fraction of non-histone proteins caused a decrease in the transcription level evaluated by the Moore method, throughout the whole postnatal ontogenesis, and no effect of extraction was noticed in respect of the AS-staining of nuclear histones.
AuthorsA V Grigor'eva, V N Iarygin
JournalTsitologiia (Tsitologiia) Vol. 27 Issue 2 Pg. 191-5 (Feb 1985) ISSN: 0041-3771 [Print] Russia (Federation)
Vernacular TitleTsitologicheskiĭ analiz belkovogo komponenta iadernogo khromatina simpaticheskikh nevrotsitov krysy v postnatal'nom ontogeneze. II. Vozrastnye izmeneniia negistonovykh iadernykh belkov, vyiavliaemye prochnym zelenym, i matrichnaia aktivnost' do i posle ékstraktsii slabosviazannoĭ fraktsii.
PMID2581342 (Publication Type: Comparative Study, English Abstract, Journal Article)
Chemical References
  • Chromosomal Proteins, Non-Histone
  • Histones
  • Lissamine Green Dyes
  • ammoniacal silver
  • Silver
  • Fast Green FCF
Topics
  • Aging
  • Animals
  • Cell Differentiation
  • Cell Nucleus (metabolism, ultrastructure)
  • Chromosomal Proteins, Non-Histone (isolation & purification, metabolism)
  • Ganglia, Sympathetic (metabolism, ultrastructure)
  • Histones (metabolism)
  • Lissamine Green Dyes
  • Neurons (metabolism, ultrastructure)
  • Nissl Bodies (metabolism, ultrastructure)
  • Rats
  • Silver
  • Staining and Labeling (methods)
  • Transcription, Genetic

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