Abstract | BACKGROUND: METHODS: LDPDT efficacy was evaluated by endoscopic, macroscopic, and histological analysis. Myeloperoxidase levels were quantified by enzyme linked immunosorbent assay and cytokines expression by quantitative RT-PCR analysis. The integrity of the intestinal barrier was evaluated by immunostaining, and bacterial composition of the fecal microbiota was determined by 454 pyrosequencing of V3-V4 region of bacterial 16S rRNA genes. RESULTS: LDPDT reduced intestinal tumor growth by decreasing the expression of a wide range of inflammatory mediators and by lowering neutrophil influx. LDPDT treatment prevents onset of a dysbiotic microbiota in the colitis-associated cancer model. CONCLUSIONS:
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Authors | Aurélie Reinhard, Aude Bressenot, Romain Dassonneville, Alexandre Loywick, David Hot, Christophe Audebert, Sophie Marchal, François Guillemin, Mathias Chamaillard, Laurent Peyrin-Biroulet, Lina Bezdetnaya |
Journal | Inflammatory bowel diseases
(Inflamm Bowel Dis)
Vol. 21
Issue 5
Pg. 985-95
(May 2015)
ISSN: 1536-4844 [Electronic] England |
PMID | 25806846
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Mesoporphyrins
- Photosensitizing Agents
- temoporfin
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Topics |
- Animals
- Colitis
(chemically induced, complications, pathology)
- Colonic Neoplasms
(etiology, pathology, prevention & control)
- Colonoscopy
- Cytokines
(metabolism)
- Enzyme-Linked Immunosorbent Assay
- Female
- Gene Expression Profiling
- Humans
- Immunoenzyme Techniques
- Lymphoma, Large B-Cell, Diffuse
(drug therapy, metabolism, pathology)
- Mesoporphyrins
(therapeutic use)
- Mice
- Mice, Inbred C57BL
- Photochemotherapy
- Photosensitizing Agents
(therapeutic use)
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