The effect of 5-azacytidine and its analogues on blast cell renewal in acute myeloblastic leukemia.

Abstract

Blast cells from patients with acute myeloblastic leukemia were exposed to 5-azacytidine (5-aza) and its analogues 5-aza 2'-deoxycytidine (5-aza-dr) and 6-azacytidine (6-aza). Simple negative exponential survival curves were obtained for the three drugs, but the sensitivity varied; 5-aza-dr was most toxic, 6-aza was least toxic, and 5-aza was intermediate. Colonies surviving drug exposure were replated; 5-aza and 5-aza-dr were found to increase secondary plating efficiency, whereas 6-aza was inactive. The findings provide indirect evidence for a role for DNA methylation in the regulation of blast cell self-renewal.

• Authors: T Motoji, T Hoang, D Tritchler, E A McCulloch
• Journal: Blood (Blood) Vol. 65 Issue 4 Pg. 894-901 (Apr 1985) ISSN: 0006-4971 [Print] United States
• PMID: 2579689 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Culture Media
• Decitabine
• Azacitidine
• 6-azacytidine

Topics

• Azacitidine (analogs & derivatives, pharmacology)
• Cell Division (drug effects)
• Culture Media
• Decitabine
• Dose-Response Relationship, Drug
• Humans
• Leukemia, Myeloid, Acute (blood, pathology)
• Neoplastic Stem Cells (drug effects)
• Phenotype
• Stem Cells (drug effects)