Doxorubicin-loaded star-shaped copolymer PLGA-vitamin E TPGS nanoparticles for lung cancer therapy.

Abstract

A doxorubicin-loaded mannitol-functionalized poly(lactide-co-glycolide)-b-D-α-tocopheryl polyethylene glycol 1000 succinate nanoparticles (DOX-loaded M-PLGA-b-TPGS NPs) were prepared by a modified nanoprecipitation method. The NPs were characterized by the particle size, surface morphology, particle stability, in vitro drug release and cellular uptake efficiency. The NPs were near-spherical with narrow size distribution. The size of M-PLGA-b-TPGS NPs was ~110.9 nm (much smaller than ~143.7 nm of PLGA NPs) and the zeta potential was -35.8 mV (higher than -42.6 mV of PLGA NPs). The NPs exhibited a good redispersion since the particle size and surface charge hardly changed during 3-month storage period. In the release medium (phosphate buffer solution vs. fetal bovine serum), the cumulative drug release of DOX-loaded M-PLGA-b-TPGS, PLGA-b-TPGS, and PLGA NPs were 76.41 versus 83.11 %, 58.94 versus 73.44 % and 45.14 versus 53.12 %, respectively. Compared with PLGA-b-TPGS NPs and PLGA NPs, the M-PLGA-b-TPGS NPs possessed the highest cellular uptake efficiency in A549 and H1975 cells (lung cancer cells). Ultimately, both in vitro and in vivo antitumor activities were evaluated. The results showed that M-PLGA-b-TPGS NPs could achieve a significantly higher level of cytotoxicity in cancer cells and a better antitumor efficiency on xenograft BALB/c nude mice tumor model than free DOX. In conclusion, the DOX-loaded M-PLGA-b-TPGS could be used as a potential DOX-loaded nanoformulation in lung cancer chemotherapy.

• Authors: Jinxie Zhang, Wei Tao, Yuhan Chen, Danfeng Chang, Teng Wang, Xudong Zhang, Lin Mei, Xiaowei Zeng, Laiqiang Huang
• Journal: Journal of materials science. Materials in medicine (J Mater Sci Mater Med) Vol. 26 Issue 4 Pg. 165 (Apr 2015) ISSN: 1573-4838 [Electronic] United States
• PMID: 25791459 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibiotics, Antineoplastic
• Delayed-Action Preparations
• Nanocapsules
• Vitamin E
• Polylactic Acid-Polyglycolic Acid Copolymer
• Polyglycolic Acid
• Lactic Acid
• Polyethylene Glycols
• Doxorubicin
• tocophersolan

Topics

• Animals
• Antibiotics, Antineoplastic (administration & dosage, chemistry)
• Apoptosis (drug effects)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Delayed-Action Preparations (administration & dosage, chemical synthesis)
• Diffusion
• Doxorubicin (administration & dosage, chemistry)
• Lactic Acid (chemistry)
• Lung Neoplasms (drug therapy, pathology)
• Mice
• Mice, Inbred BALB C
• Nanocapsules (administration & dosage, chemistry)
• Particle Size
• Polyethylene Glycols (chemistry)
• Polyglycolic Acid (chemistry)
• Polylactic Acid-Polyglycolic Acid Copolymer
• Treatment Outcome
• Vitamin E (analogs & derivatives, chemistry)