B5, a new B cell-restricted activation antigen.

Abstract

The characterization of a new human B cell-restricted activation antigen (B5) is described in this report. With the use of a monoclonal antibody to B5, we show that B5 can be detected on peripheral blood or splenic B cells after 1 day of stimulation with either anti-immunoglobulin, protein A, Epstein Barr virus, or pokeweed mitogen. In contrast, B5 was not expressed on resting B, T, or myeloid cells. More important, B5 could not be detected on activated T cells or monocytes. The B5 antigen was expressed on some lymphoblastoid B cell lines and B cell neoplasms but was not expressed on leukemias or lymphomas of T or myeloid origin. The B5 antigen is distinct from previously reported B cell activation antigens by its m.w. and pattern of cellular expression. These studies suggest that B5 is a novel B cell-restricted activation antigen, which may be useful to study the events of early human B cell activation.

• Authors: A S Freedman, A W Boyd, K C Anderson, D C Fisher, S F Schlossman, L M Nadler
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 134 Issue 4 Pg. 2228-35 (Apr 1985) ISSN: 0022-1767 [Print] United States
• PMID: 2579134 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antigens, Differentiation, B-Lymphocyte
• Antigens, Neoplasm
• Antigens, Surface
• Epitopes

Topics

• Animals
• Antibodies, Monoclonal (biosynthesis)
• Antigen-Antibody Reactions
• Antigens, Differentiation, B-Lymphocyte
• Antigens, Neoplasm (analysis, immunology)
• Antigens, Surface (immunology, isolation & purification)
• B-Lymphocytes (immunology)
• Bone Marrow (immunology)
• Cell Line
• Epitopes (analysis)
• Female
• Humans
• Hybridomas (metabolism)
• Lymphocyte Activation
• Lymphoid Tissue (immunology)
• Mice
• Mice, Inbred BALB C
• Spleen (cytology)