HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Proteomics identification of novel fibrinogen-binding proteins of Streptococcus suis contributing to antiphagocytosis.

Abstract
Streptococcus suis serotype 2 (SS2) induced sepsis and meningitis are often accompanied by bacteremia. However, the mechanism whereby it helps S. suis to evade PMN-mediated phagocytosis remain unclear. Because of the central roles of bacteria-human fibrinogen (hFg) interaction in innate immunity, here, a proteomics based Far-western blotting (PBFWB) was developed to identify the fibrinogen-binding surface proteins of S. suis (SsFBPs) on a large-scale. And then thirteen potential SsFBPs were identified by PBFWB and we selected seven potential surface proteins to further confirm their binding ability to hFg, of which the gene mutant strains of MRP displayed significantly decrease in binding to immobilized hFg. Additionally, the polyclonal antibodies against Enolase were found to significantly inhibit the binding of SS2 to hFg. Strikingly, MRP and Enolase were found to improve the antiphagocytic ability of SS2 to PMNs by interacting with hFg and enhance the survival of SS2 in human blood. Taken together, the PBFWB method provides useful clues to the bacteria-host interactions. These studies firstly disclose MRP and Enolase were involved in immune evasion of SS2 at least in part by binding to Fg, which make them potential targets for therapies for SS2 infection.
AuthorsYaya Pian, Pingping Wang, Peng Liu, Yuling Zheng, Li Zhu, Hengliang Wang, Bin Xu, Yuan Yuan, Yongqiang Jiang
JournalFrontiers in cellular and infection microbiology (Front Cell Infect Microbiol) Vol. 5 Pg. 19 ( 2015) ISSN: 2235-2988 [Electronic] Switzerland
PMID25789245 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Bacterial Proteins
  • Carrier Proteins
  • Membrane Proteins
  • Mrp22 protein, Streptococcus
  • Fibrinogen
  • Phosphopyruvate Hydratase
Topics
  • Bacterial Proteins (chemistry, genetics, immunology, metabolism)
  • Carrier Proteins (chemistry, genetics, immunology, metabolism)
  • Electrophoresis, Gel, Two-Dimensional
  • Fibrinogen (genetics, immunology, metabolism)
  • Host-Pathogen Interactions
  • Humans
  • Immune Evasion
  • Mass Spectrometry
  • Membrane Proteins (chemistry, genetics, immunology, metabolism)
  • Molecular Sequence Data
  • Phagocytosis
  • Phosphopyruvate Hydratase (chemistry, genetics, immunology, metabolism)
  • Protein Binding
  • Proteomics
  • Streptococcal Infections (immunology, metabolism, microbiology)
  • Streptococcus suis (chemistry, genetics, immunology, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: