Abstract |
Breast cancer (BC) is the most frequently diagnosed type of cancer all over the world. Angiogenesis, a physiological or pathological process characterized by the sprouting of new blood vessels from existing vessels, plays a vital role in tumor nutrition. In this work, we used JSI-124 ( Cucurbitacin I), a selective JAK/STAT3 signaling pathway inhibitor, to investigate the role of STAT3 in tumor angiogenesis of a human BC cell line in vitro. JSI-124 inhibited cell viability, proliferation, adhesion, migration and tube formation of a human BC cell line MDA-MB-468. After transfection with pMXs-Stat3C, a dominant active mutant, the inhibitory effects of JSI-124 on MDA-MB-468 were abolished. Furthermore, JSI-124 reduced the phosphorylation of STAT3. These results suggested that JSI-124 inhibited tumor angiogenesis of the human BC cell line in vitro through the reduction of STAT3 phosphorylation. In addition, JSI-124 could reduce VEGF transcription and secretion, suggesting that JSI-124 is also involved in the inhibition of the VEGF autocrine loop in the tumor microenvironment.
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Authors | Jia Qi, Ge Xia, Cheng Rong Huang, Jian Xia Wang, Jian Zhang |
Journal | The American journal of Chinese medicine
(Am J Chin Med)
Vol. 43
Issue 2
Pg. 337-47
( 2015)
ISSN: 1793-6853 [Electronic] Singapore |
PMID | 25787299
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- STAT3 Transcription Factor
- STAT3 protein, human
- Triterpenes
- Vascular Endothelial Growth Factor A
- cucurbitacin I
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Topics |
- Autocrine Communication
(drug effects)
- Breast Neoplasms
(blood supply, genetics, pathology)
- Cell Adhesion
(drug effects)
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Cell Survival
(drug effects)
- Cell Transformation, Neoplastic
(drug effects)
- Depression, Chemical
- Female
- Humans
- Molecular Targeted Therapy
- Neovascularization, Pathologic
(drug therapy)
- Phosphorylation
(drug effects)
- Phytotherapy
- STAT3 Transcription Factor
(metabolism, physiology)
- Signal Transduction
(drug effects)
- Transcription, Genetic
(drug effects)
- Triterpenes
(pharmacology, therapeutic use)
- Tumor Microenvironment
(drug effects, genetics)
- Vascular Endothelial Growth Factor A
(genetics)
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