HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Canolol inhibits gastric tumors initiation and progression through COX-2/PGE2 pathway in K19-C2mE transgenic mice.

Abstract
4-Vinyl-2, 6-dimethoxyphenol (canolol) is an antioxidant phenolic compound extracted from crude canola oil. In current research, K19-C2mE transgenic mice, developing hyperplastic tumors spontaneously in the glandular stomach, were used to study the mechanisms involved in the anti-inflammation and anti-tumor effects of canolol. Tg mice receiving canolol diet had a reduced tumor incidence, to 41.2%, compared with Non-treatment Tg mice, 77.8% of which had gastric tumor (P=0.002). Besides that, the mean tumor diameter was decreased from 6.5 mm to 4.5 mm (P<0.001) after canolol administration. COX-2/PGE2 pathway is known to play pivotal role in inflammation-induced gastric tumorigenesis. The neutrophils and lymphocytes infiltration was suppressed significantly, and the mRNA levels of the proinflammatory cytokines COX-2, IL-1β and IL-12b were also downregulated in gastric mucosa. Additionally, immunohistochemical analysis showed that COX-2, EP2, Gαs and β-catenin, key factors involving in PGE2 signal transduction, were positive staining with higher H scores in Non-treatment Tg mice, while the expressions were suppressed significantly by 0.1% canolol (P<0.001). In addition, tumor-suppressor miR-7 was reactivated after canolol administration, and COX-2 was showed to be a functional target of miR-7 to suppress the tumor progression. In conclusion, canolol could inhibit the gastritis-related tumor initiation and progression, and the suppression effect was correlated with the blocking up of canonical COX-2/PGE2 signaling pathway and might be regulated by miR-7.
AuthorsDonghui Cao, Jing Jiang, Tetsuya Tsukamoto, Ruming Liu, Lin Ma, Zhifang Jia, Fei Kong, Masanobu Oshima, Xueyuan Cao
JournalPloS one (PLoS One) Vol. 10 Issue 3 Pg. e0120938 ( 2015) ISSN: 1932-6203 [Electronic] United States
PMID25781635 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • IL1B protein, mouse
  • Interleukin-12 Subunit p40
  • Interleukin-1beta
  • MIRN7 microRNA, mouse
  • MicroRNAs
  • Neoplasm Proteins
  • Phenols
  • RNA, Neoplasm
  • Vinyl Compounds
  • Ptgs2 protein, mouse
  • Cyclooxygenase 2
  • Dinoprostone
  • 4-vinyl-2,6-dimethoxyphenol
Topics
  • Animals
  • Cyclooxygenase 2 (genetics, metabolism)
  • Dinoprostone (genetics, metabolism)
  • Interleukin-12 Subunit p40
  • Interleukin-1beta (genetics, metabolism)
  • Mice
  • Mice, Transgenic
  • MicroRNAs (genetics, metabolism)
  • Neoplasm Proteins (genetics, metabolism)
  • Phenols (pharmacology)
  • RNA, Neoplasm (genetics, metabolism)
  • Signal Transduction (drug effects, genetics)
  • Stomach Neoplasms (genetics, metabolism, pathology, prevention & control)
  • Vinyl Compounds (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: