Nitrogen starvation acclimation in non-diazotrophic cyanobacteria is characterized by a process termed
chlorosis, where the light harvesting pigments are degraded and the cells gradually tune down photosynthetic and metabolic activities. The
chlorosis response is governed by a complex and poorly understood regulatory network, which converges at the expression of the nblA gene, the triggering factor for
phycobiliprotein degradation. This study established a method that allows uncoupling metabolic and redox-signals involved in
nitrogen-
starvation acclimation. Inhibition of
glutamine synthetase (GS) by a precise dosage of
l-methionine-sulfoximine (MSX) mimics the metabolic situation of
nitrogen starvation. Addition of
nitrate to such MSX-inhibited cells eliminates the associated redox-stress by enabling electron flow towards
nitrate/
nitrite reduction and thereby, prevents the induction of nblA expression and the associated
chlorosis response. This study demonstrates that
nitrogen starvation is perceived not only through metabolic signals, but requires a redox signal indicating over-reduction of PSI-reduced electron acceptors. It further establishes a cryptic role of
nitrate/
nitrite reductases as electron sinks to balance conditions of over-reduction.