Abstract | OBJECTIVES: METHODS: Blood from each donor was subjected to 62 different handling conditions prior to complement assays. RESULTS:
Complement biomarkers (C4d/C3a/factor Bb/C5a/C5b-9) are stable at room temperature (RT) for up to 4 hours in whole blood containing citrate or EDTA. However, under similar conditions, levels of C4d and C3a were significantly higher in serum than those in plasma. Thawing of the samples on ice or at RT had no significant effect on complement levels. In contrast, thawing at 37°C resulted in striking increases in levels of the complement system in serum and citrated plasma but not in EDTA plasma. Up to four freeze/thaw cycles on ice or RT did not substantially increase the levels of C3a, factor Bb, C5a, and C5b-9 but had a significant effect on C4d. Long-term storage of citrated plasma at -80°C for up to 6 years had no significant effect on levels of complement factors. CONCLUSIONS: The results from this study thus provide crucial guidelines for future investigations using complement biomarkers to define the role of complement system in disease.
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Authors | Shangbin Yang, Michael McGookey, Yi Wang, Spero R Cataland, Haifeng M Wu |
Journal | American journal of clinical pathology
(Am J Clin Pathol)
Vol. 143
Issue 4
Pg. 558-65
(Apr 2015)
ISSN: 1943-7722 [Electronic] England |
PMID | 25780008
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright© by the American Society for Clinical Pathology. |
Chemical References |
- Biomarkers
- Complement System Proteins
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Topics |
- Biomarkers
(analysis, metabolism)
- Blood Preservation
(methods)
- Blood Specimen Collection
(methods)
- Complement Activation
- Complement System Proteins
(analysis, metabolism)
- Humans
- Specimen Handling
(methods)
- Temperature
- Thrombotic Microangiopathies
(blood, immunology)
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