Abstract | UNLABELLED:
D-Glutamate is an essential component of bacterial peptidoglycan and a building block of the poly-γ-D- glutamic acid (PDGA) capsule of Bacillus anthracis, the causative agent of anthrax. Earlier work suggested that two glutamate racemases, encoded by racE1 and racE2, are each essential for growth of B. anthracis, supplying D- glutamic acid for the synthesis of peptidoglycan and PDGA capsule. Earlier work could not explain, however, why two enzymes that catalyze the same reaction may be needed for bacterial growth. Here, we report that deletion of racE1 or racE2 did not prevent growth of B. anthracis Sterne (pXO1(+) pXO2(-)), the noncapsulating vaccine strain, or of B. anthracis Ames (pXO1(+) pXO2(+)), a fully virulent, capsulating isolate. While mutants with deletions in racE1 and racE2 were not viable, racE2 deletion delayed vegetative growth of B. anthracis following spore germination and caused aberrant cell shapes, phenotypes that were partially restored by exogenous D-glutamate. Deletion of racE1 or racE2 from B. anthracis Ames did not affect the production or stereochemical composition of the PDGA capsule. A model is presented whereby B. anthracis, similar to Bacillus subtilis, utilizes two functionally redundant racemase enzymes to synthesize D- glutamic acid for peptidoglycan synthesis. IMPORTANCE:
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Authors | So-Young Oh, Stefan G Richter, Dominique M Missiakas, Olaf Schneewind |
Journal | Journal of bacteriology
(J Bacteriol)
Vol. 197
Issue 11
Pg. 1854-61
(Jun 2015)
ISSN: 1098-5530 [Electronic] United States |
PMID | 25777674
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | Copyright © 2015, American Society for Microbiology. All Rights Reserved. |
Chemical References |
- Bacterial Proteins
- poly(gamma-glutamic acid)
- Polyglutamic Acid
- Glutamic Acid
- Amino Acid Isomerases
- glutamate racemase
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Topics |
- Amino Acid Isomerases
(genetics, metabolism)
- Bacillus anthracis
(enzymology, genetics, growth & development)
- Bacterial Proteins
(genetics, metabolism)
- Gene Deletion
- Glutamic Acid
(metabolism)
- Polyglutamic Acid
(analogs & derivatives, metabolism)
- Sequence Deletion
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