p63 Expression in the Gerbil Hippocampus Following Transient Ischemia and Effect of Ischemic Preconditioning on p63 Expression in the Ischemic Hippocampus.

Abstract

p63 is a transcription factor of p53 gene family, which are involved in development, differentiation and cell response to stress; however, its roles in ischemic preconditioning (IPC) in the brain are not clear. In the present study, we investigated the effect of IPC on p63 immunoreactivity caused by 5 min of transient cerebral ischemia in gerbils. IPC was induced by subjecting the gerbils to 2 min of transie ischemia 1 day prior to 5 min of transient ischemia. The animals were randomly assigned to four groups (sham-operated-group, ischemia-operated-group, IPC plus (+)-sham-operated-group and IPC + ischemia-operated-group). The number of viable neurons in the stratum pyramidale of the hippocampal CA1 region (CA1) was significantly increased by IPC + ischemia-operated-group compared with that in the ischemia-operated-group 5 days after ischemic insult. We found that strong p63 immunoreactivity was detected in the CA1 pyramidal neurons in the sham-operated-group, and the immunoreactivity was decreased with time after ischemia-reperfusion. In addition, strong p63 immunoreactivity was newly expressed in microglial cells of the CA1 region from 2 days after ischemia-reperfusion. In all the IPC + sham-operated-groups, p63 immunoreactivity in the CA1 pyramidal neurons was similar to that in the sham-operated-group, and the immunoreactivity was well maintained in the IPC + ischemia-operated-groups after cerebral ischemia. In brief, our present findings show that IPC dramatically protected the reduction of p63 immunoreactivity in the pyramidal neurons of the CA1 region after ischemia-reperfusion, and this result suggests that the expression of p63 may be necessary for neurons to survive after transient cerebral ischemia.

• Authors: Jae-Chul Lee, Geum-Sil Cho, In Hye Kim, Joon Ha Park, Jeong-Hwi Cho, Ji Hyeon Ahn, Eun Joo Bae, Ji Yun Ahn, Chan Woo Park, Jun Hwi Cho, Young-Myeong Kim, Moo-Ho Won, Hui Young Lee
• Journal: Neurochemical research (Neurochem Res) Vol. 40 Issue 5 Pg. 1013-22 (May 2015) ISSN: 1573-6903 [Electronic] United States
• PMID: 25777256 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Phosphoproteins
• Trans-Activators
• Trp63 protein, mouse

Topics

• Animals
• CA1 Region, Hippocampal (metabolism, pathology)
• Gene Expression Regulation
• Gerbillinae
• Ischemic Attack, Transient (metabolism, pathology, prevention & control)
• Ischemic Preconditioning (methods)
• Male
• Phosphoproteins (biosynthesis)
• Trans-Activators (biosynthesis)