Abstract | BACKGROUND: The aim of this study was to evaluate the histological and immunohistochemical effects of levosimendan on liver injury induced by myocardial ischemia and reperfusion (I/R) in a rat model. METHODS: Twenty-four male Wistar Albino rats were randomly divided into the four groups: Group C (Control, n = 6), Group I/R (n = 6), Group BI (I/R group treated with levosimendan before ischemia, n = 6), and Group AI (I/R group treated with levosimendan after ischemia, n = 6). Myocardial I/R was induced by ligation of the left anterior descending coronary artery for 30 min followed by two hours of reperfusion in I/R and I/R+Levosimendan groups. At the end of the study, liver tissue samples were obtained for histopathological and immunohistochemical examination. RESULTS: Masson Trichrome staining revealed significant hepatocyte degeneration and necrosis most marked in portal acinus Zone 3, especially around the central veins in Group I/R. Histopathological changes in Group AI were more similar to the changes in Group I/R. Milder hepatocellular degeneration was found in Group BI, when compared to groups I/R and AI. Immunohistochemical score was found to be significantly higher in Group I/R compared to groups C, BI and AI (p < 0.0001). The scores in groups BI and AI were found to be similar (p = 0.068). CONCLUSION:
Levosimendan ameliorates liver injury induced by myocardial IR, especially when administered before induction of ischemia (Fig. 9, Ref. 37).
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Authors | G L Oktar, N Demir Amac, C Elmas, M Arslan, G Goktas, E Iriz, D Erer, M H Zor, T Tatar |
Journal | Bratislavske lekarske listy
(Bratisl Lek Listy)
Vol. 116
Issue 4
Pg. 241-7
( 2015)
ISSN: 0006-9248 [Print] Slovakia |
PMID | 25773952
(Publication Type: Journal Article)
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Chemical References |
- Hydrazones
- Pyridazines
- Vasodilator Agents
- Simendan
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Topics |
- Acute Lung Injury
(etiology, pathology, prevention & control)
- Animals
- Disease Models, Animal
- Hydrazones
(pharmacology)
- Liver
(drug effects, pathology)
- Male
- Myocardial Ischemia
(complications, drug therapy)
- Myocardial Reperfusion Injury
(complications, drug therapy)
- Pyridazines
(pharmacology)
- Rats
- Rats, Wistar
- Simendan
- Vasodilator Agents
(pharmacology)
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