Immunohistochemistry, histopathology, and biomarker studies of swertiamarin, a secoiridoid glycoside, prevents and protects streptozotocin-induced β-cell damage in Wistar rat pancreas.
Abstract | BACKGROUND: OBJECTIVES: The present study aims at the following: (1) to evaluate antidiabetic, anti-hyperlipidaemic, activity of swertiamarin in Streptozotocin- induced diabetic rats using biomarkers. (2) To assess histopathological alterations in Pancreas, Liver, Kidney, and Heart of swertiamarin-treated STZ-induced diabetic rats and confirm cytoprotective activity of swertiamarin by Immunohistochemistry and morphometric investigations. METHODS: Diabetes was induced intraperitoneally in male Wistar rats by Streptozotocin (STZ 50 mg/kg). After STZ-induction, hyperglycemic rats were treated with doses of swertiamarin orally (15, 25, 50 mg/kg) each for 28 days. Glibenclamide (2.5 mg/kg), a sulphonyl urea, was used as a standard drug. The glycemic control was measured by the biochemical parameter assays. Histopathology analysis of organs and immunohistochemistry of islets were carried out. RESULTS: Our study results showed that oral administration of swertiamarin at a dosage of 15, 25, 50 mg/kg bw for 28 days resulted in a significant (p < 0.01) decrease in fasting blood glucose, HbA1c, TC, TG, LDL, and increased the levels of hemoglobin, plasma insulin, TP, body weight, and HDL levels significantly (p < 0.01) when compared to STZ-induced diabetic rats, as confirmed by immunohistochemical studies. The effect of swertiamarin on Carbohydrate-metabolizing enzymes was investigated and found to have normal therapeutic activity. Histopathological studies of Pancreas of swertiamarin-treated diabetic rats showed regeneration of islets when compared to STZ-induced diabetic rats, as confirmed by immunohistochemical studies. CONCLUSION:
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Authors | G Dhanavathy |
Journal | Journal of endocrinological investigation
(J Endocrinol Invest)
Vol. 38
Issue 6
Pg. 669-84
(Jun 2015)
ISSN: 1720-8386 [Electronic] Italy |
PMID | 25770453
(Publication Type: Journal Article)
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Chemical References |
- Blood Glucose
- Hypoglycemic Agents
- Iridoid Glucosides
- Plant Extracts
- Protective Agents
- Pyrones
- swertiamarin
- Glyburide
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Topics |
- Animals
- Blood Glucose
(metabolism)
- Diabetes Mellitus, Experimental
(metabolism, pathology, prevention & control)
- Glyburide
(pharmacology, therapeutic use)
- Heart
(drug effects)
- Hypoglycemic Agents
(pharmacology, therapeutic use)
- Immunohistochemistry
- Iridoid Glucosides
(pharmacology, therapeutic use)
- Islets of Langerhans
(drug effects, metabolism, pathology)
- Kidney
(drug effects, metabolism, pathology)
- Liver
(drug effects, metabolism, pathology)
- Male
- Myocardium
(metabolism, pathology)
- Pancreas
(drug effects, metabolism, pathology)
- Plant Extracts
(pharmacology, therapeutic use)
- Protective Agents
(pharmacology, therapeutic use)
- Pyrones
(pharmacology, therapeutic use)
- Rats
- Rats, Wistar
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