Catalase is needed to avoid tissue peroxidation in Rana perezi in normoxia.

Abstract

1. In order to clarify the relative role of catalase (CAT) and glutathione peroxidases (GSH-Px) at normal and high O2 tensions, Rana perezi frogs were chronically treated with aminotriazole (AT), hyperoxia, or both. 2. A 100% survival was observed with both treatments. Hyperoxia increased liver catalase and kidney TBA-RS and decreased GSH-Px. 3. AT caused quantitatively higher alterations than hyperoxia in both organs: CAT was depleted, TBA-RS increased (114% in kidney) and GSH-Px decreased. 4. It is concluded that in Rana perezi (a) CAT, in spite of its much higher KM and Vmax in relation to GSH-Px, is needed to avoid oxidative stress even in normoxia; (b) normoxic tissues have significative amounts of H2O2; (c) GSH-Px does not compensate the lack of CAT.

• Authors: G Barja de Quiroga, M López-Torres, R Pérez-Campo
• Journal: Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology (Comp Biochem Physiol C Comp Pharmacol Toxicol) Vol. 94 Issue 2 Pg. 391-8 ( 1989) ISSN: 0742-8413 [Print] England
• PMID: 2576777 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Peroxides
• Thiobarbiturates
• Catalase
• Glutathione Peroxidase
• thiobarbituric acid
• Oxygen
• Amitrole

Topics

• Amitrole (pharmacology)
• Animals
• Catalase (antagonists & inhibitors, physiology)
• Glutathione Peroxidase (physiology)
• Kidney (enzymology)
• Liver (enzymology)
• Oxidation-Reduction
• Oxygen (pharmacology)
• Peroxides (metabolism)
• Ranidae
• Thiobarbiturates