The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1.

Abstract

The altered expression of the SOX2 transcription factor is associated with oncogenic or tumor suppressor functions in human cancers. This factor regulates the migration and invasion of different cancer cells. In this study we investigated the effect of constitutive SOX2 overexpression on the migration and adhesion capacity of embryonal teratocarcinoma NT2/D1 cells derived from a metastasis of a human testicular germ cell tumor. We detected that increased SOX2 expression changed the speed, mode and path of cell migration, but not the adhesion ability of NT2/D1 cells. Additionally, we demonstrated that SOX2 overexpression increased the expression of the tumor suppressor protein p53 and the HDM2 oncogene. Our results contribute to the better understanding of the effect of SOX2 on the behavior of tumor cells originating from a human testicular germ cell tumor. Considering that NT2/D1 cells resemble cancer stem cells in many features, our results could contribute to the elucidation of the role of SOX2 in cancer stem cells behavior and the process of metastasis.

• Authors: Danijela Drakulic, Jelena Marjanovic Vicentic, Marija Schwirtlich, Jelena Tosic, Aleksandar Krstic, Andrijana Klajn, Milena Stevanovic
• Journal: Anais da Academia Brasileira de Ciencias (An Acad Bras Cienc) Vol. 87 Issue 1 Pg. 389-404 (Mar 2015) ISSN: 1678-2690 [Electronic] Brazil
• PMID: 25761220 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• SOX2 protein, human
• SOXB1 Transcription Factors

Topics

• Cell Adhesion (physiology)
• Cell Line, Tumor
• Cell Movement (physiology)
• Cell Proliferation
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry
• Male
• SOXB1 Transcription Factors (metabolism)
• Teratocarcinoma (metabolism, pathology)
• Tissue Array Analysis