Effects of electrophotodynamic therapy in vitro on human melanoma cells--melanotic (MeWo) and amelanotic (C32).

Abstract

Photodynamic therapy has been considered ineffective for melanomas because of the competition between the absorbance of melanin from the melanoma and the absorbance of photosensitizers at the photosensitizer excitation light wavelength. Melanomas show considerable heterogeneity and resistance to phototherapy. The effectiveness of photodynamic therapy could be intensified by electroporation for enhanced transport of a photosensitizer by transient pores in the membrane. In this study, photodynamic therapy combined with electroporation was tested in vitro on the human melanoma cell lines melanotic melanoma (MeWo) and amelanotic melanoma (C32). Control experiments were conducted on human keratinocytes (HaCaT). Photofrin was used as a photosensitizer. Photosensitizer distribution, cloning efficacy test, comet assay, and assessment of apoptotic proteins were performed. Melanin levels were determined before and after photodynamic therapy. The experiments indicated that electroporation effectively supports the photodynamic method. It was found that photodynamic therapy with electroporation efficiently induces apoptosis in melanotic and amelanotic melanoma cells.

• Authors: Anna Choromanska, Julita Kulbacka, Nina Rembialkowska, Justyna Pilat, Małgorzata Drag-Zalesinska, Teresa Wysocka, Arnold Garbiec, Malgorzata Kotulska, Jolanta Saczko
• Journal: Melanoma research (Melanoma Res) Vol. 25 Issue 3 Pg. 210-24 (Jun 2015) ISSN: 1473-5636 [Electronic] England
• PMID: 25756554 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Melanins
• Neoplasm Proteins
• Photosensitizing Agents
• Dihematoporphyrin Ether
• Caspases

Topics

• Absorption, Physiological (radiation effects)
• Antineoplastic Agents (adverse effects, metabolism, pharmacology)
• Apoptosis (drug effects, radiation effects)
• Caspases (metabolism)
• Cell Line, Transformed
• Cell Line, Tumor
• Comet Assay
• DNA Damage
• Dihematoporphyrin Ether (adverse effects, metabolism, pharmacology)
• Electrochemotherapy (adverse effects)
• Electroporation
• Humans
• Keratinocytes (cytology, drug effects, metabolism, radiation effects)
• Melanins (metabolism)
• Melanoma (drug therapy, metabolism, pathology)
• Melanoma, Amelanotic (drug therapy, metabolism, pathology)
• Neoplasm Proteins (metabolism)
• Photochemotherapy (adverse effects)
• Photosensitizing Agents (adverse effects, metabolism, pharmacology)
• Time Factors