Fibroblast growth factor receptor 2 is associated with poor overall survival in clear cell carcinoma of the ovary and may be a novel therapeutic approach.

Abstract

OBJECTIVE:

We previously found that gene and protein expression of fibroblast growth factor receptor (FGFR) 2 were increased in ovarian clear cell carcinoma (CCC); here, we examined FGFR2 expression in CCC tumor tissues and its correlation with clinical parameters. We also analyzed the effect of an FGFR inhibitor on the growth of CCC cells to investigate whether FGFR2 could be a therapeutic target for this disease.

METHODS:

We analyze the protein expression of FGFR2 by immunohistochemical staining in CCC from 112 patients and evaluated the association of these molecular parameters with clinical outcome. We treated the 11 CCC cell lines with an FGFR inhibitor, and then assessed cell viability, the expression of protein in FGFR2 signaling pathway, and cell cycle distribution.

RESULTS:

The expressions of FGFR2 were found in 96% of CCC. The 5-year survival rate for patients with a moderate or strong expression of FGFR2 was significantly lower than that for those with an absent or poor expression of FGFR2 (54% vs 79%). Multivariable analysis revealed that FGFR2 expression and disease stage were independent prognostic factors. The FGFR inhibitor effectively suppressed the growth of CCC cells with induction of G1 cell cycle arrest and down-regulated the expression of phosphorylated Akt and phosphorylated ERK.

CONCLUSIONS:

FGFR2 is an important biomarker predictive of patient outcome and is a potential target for CCC. Further study is warranted for FGFR inhibitor to treat CCC.

• Authors: Hiroaki Itamochi, Nao Oumi, Tetsuro Oishi, Fuminori Taniguchi, Tadahiro Shoji, Hiroyuki Fujiwara, Toru Sugiyama, Mitsuaki Suzuki, Junzo Kigawa, Tasuku Harada
• Journal: International journal of gynecological cancer : official journal of the International Gynecological Cancer Society (Int J Gynecol Cancer) Vol. 25 Issue 4 Pg. 570-6 (May 2015) ISSN: 1525-1438 [Electronic] England
• PMID: 25756405 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• FGFR2 protein, human
• Receptor, Fibroblast Growth Factor, Type 2

Topics

• Adenocarcinoma, Clear Cell (metabolism, mortality, pathology)
• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (metabolism)
• Blotting, Western
• Cell Cycle
• Cell Proliferation
• Female
• Flow Cytometry
• Follow-Up Studies
• Gene Expression Regulation, Neoplastic
• Humans
• Immunoenzyme Techniques
• Middle Aged
• Neoplasm Staging
• Ovarian Neoplasms (metabolism, mortality, pathology)
• Prognosis
• Receptor, Fibroblast Growth Factor, Type 2 (metabolism)
• Survival Rate
• Tumor Cells, Cultured