Abstract | BACKGROUND: METHODS: In BALB/c mice, acute colitis was induced by intra- rectal administration of one dose TNBS, while chronic colitis was induced by administration of TNBS once a week for 7 weeks. To assess the effects of complete loss of Cav-1, Cav-1 knockout (Cav-1-/-) and control wild-type C57 mice received one TNBS administration. Body weight and clinical scores were monitored. Colon Cav-1 and pro-inflammatory cytokine levels were quantified through ELISAs. Inflammation was evaluated through histological analysis. RESULTS: Colon Cav-1 levels were significantly decreased in TNBS-induced colitis mice when compared to normal mice and also inversely correlated with colon inflammation scores and proinflammatory cytokine levels (IL-17, IFN-γ and TNF) significantly. Furthermore, after administration of TNBS, Cav-1-/- mice showed significantly increased clinical and colon inflammatory scores and body weight loss when compared with control mice. CONCLUSIONS AND SIGNIFICANCE: Cav-1 may play a protective role in the development of TNBS-induced colitis. Our findings raise an important issue in the evaluation of specific molecules in animal models that different models may exhibit opposite results because of the different mechanisms involved.
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Authors | Carolyn R Weiss, Qingdong Guan, Yanbing Ma, Gefei Qing, Charles N Bernstein, Richard J Warrington, Zhikang Peng |
Journal | PloS one
(PLoS One)
Vol. 10
Issue 3
Pg. e0119004
( 2015)
ISSN: 1932-6203 [Electronic] United States |
PMID | 25756273
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Caveolin 1
- Cytokines
- Trinitrobenzenesulfonic Acid
- Collagen
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Topics |
- Animals
- Caveolin 1
(physiology)
- Colitis
(chemically induced, immunology, metabolism)
- Collagen
(metabolism)
- Cytokines
(metabolism)
- Female
- Male
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Mice, Knockout
- Trinitrobenzenesulfonic Acid
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