Indian Sarsaparilla (Hemidesmus indicus R. Br.) is widely used in Indian
traditional medicine. In the present work, we explored the effects of decoction, traditional Ayurvedic preparation, and hydroalcoholic extract, a phytocomplex more traditionally studied and commercialized as food supplement in western medicine, from the roots as possible source of chemicals with new functional potential linked to their nutritional uses. The antiproliferative and
antioxidant properties were assayed. To test antiproliferative affects, different
cancer cell lines, growing both as monolayers (CaCo2, MCF-7, A549, K562, MDA-MB-231, Jurkat, HepG2, and LoVo) and in
suspension (K562 and Jurkat) were used. The decoction showed strong activity on HepG2 cells, while the hydroalcoholic extracts were active on HepG2, LoVo, MCF-7, K562, and Jurkat cell lines. Weak inhibition of
cancer cell proliferation was observed for the principal constituents of the preparations:
2-hydroxy-4-methoxybenzaldehyde, 2-hydroxy-4-methoxybenzoic
acid, and 3-hydroxy-4-methoxybenzaldehyde that were tested alone. The antiradical activity was tested with
2,2-diphenyl-1-picrylhydrazyl and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium
salt tests and inhibition of
nitric oxide production in
lipopolysaccharide-stimulated RAW 264.7 macrophages. Interesting result has also been obtained for hydroalcoholic extract regarding genoprotective potential (58.79% of inhibition at 37.5 µg/mL).