HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

APOBEC3B: a potential factor suppressing growth of human hepatocellular carcinoma cells.

AbstractBACKGROUND:
To realize the role of apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B (APOBEC3B) in hepatocellular carcinoma (HCC) occurrence, mRNAs of APOBEC3B from tumor and non-tumor tissues of patients with hepatectomy were isolated and in vitro studies were designed.
MATERIALS AND METHODS:
Seventy-two tumor and non-tumor tissue samples, as well as clinical data, were collected from HCC patients during hepatectomy. The mRNA of APOBEC3B was assessed by real-time polymerase chain reaction. The viability of pLV-APOBEC3B-transfected Hep 3B cells was then determined. Cell growth of pLV-APOBEC3B-transfected Hep 3B cells was evaluated by in vitro migration assay.
RESULTS:
The real-time polymerase chain reaction results indicated a higher expression of APOBEC3B mRNA in tumor tissues than in non-tumor tissues of patients with HBsAg+ HCC. The expression of APOBEC3B in tumor or non-tumor tissue was not found to be a risk factor of recurrence in patients with HCC. The cell viability assay results indicated the growth-inhibitory effects of APOBEC3B on Hep 3B cells. The cell migration results indicated that APOBEC3B inhibits wound healing in Hep 3B cells.
CONCLUSION:
Based on these observations, we infer that APOBEC3B is a potential factor contributing to suppression of tumor growth in HCC.
AuthorsPei-Fung Wu, Yaw-Sen Chen, Ting-Yin Kuo, Hsi-Hsun Lin, Ching-Wen Liu, Li-Ching Chang
JournalAnticancer research (Anticancer Res) Vol. 35 Issue 3 Pg. 1521-7 (Mar 2015) ISSN: 1791-7530 [Electronic] Greece
PMID25750306 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.
Chemical References
  • Minor Histocompatibility Antigens
  • RNA, Messenger
  • APOBEC3B protein, human
  • Cytidine Deaminase
Topics
  • Carcinoma, Hepatocellular (pathology)
  • Cell Line, Tumor
  • Cell Movement
  • Cytidine Deaminase (genetics, physiology)
  • Hepatectomy
  • Humans
  • Liver Neoplasms (pathology)
  • Minor Histocompatibility Antigens
  • Neoplasm Recurrence, Local (etiology)
  • RNA, Messenger (analysis)
  • Wound Healing

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: