Abstract | BACKGROUND: MATERIALS AND METHODS: Seventy-two tumor and non- tumor tissue samples, as well as clinical data, were collected from HCC patients during hepatectomy. The mRNA of APOBEC3B was assessed by real-time polymerase chain reaction. The viability of pLV-APOBEC3B-transfected Hep 3B cells was then determined. Cell growth of pLV-APOBEC3B-transfected Hep 3B cells was evaluated by in vitro migration assay. RESULTS: The real-time polymerase chain reaction results indicated a higher expression of APOBEC3B mRNA in tumor tissues than in non- tumor tissues of patients with HBsAg+ HCC. The expression of APOBEC3B in tumor or non- tumor tissue was not found to be a risk factor of recurrence in patients with HCC. The cell viability assay results indicated the growth-inhibitory effects of APOBEC3B on Hep 3B cells. The cell migration results indicated that APOBEC3B inhibits wound healing in Hep 3B cells. CONCLUSION: Based on these observations, we infer that APOBEC3B is a potential factor contributing to suppression of tumor growth in HCC.
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Authors | Pei-Fung Wu, Yaw-Sen Chen, Ting-Yin Kuo, Hsi-Hsun Lin, Ching-Wen Liu, Li-Ching Chang |
Journal | Anticancer research
(Anticancer Res)
Vol. 35
Issue 3
Pg. 1521-7
(Mar 2015)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 25750306
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved. |
Chemical References |
- Minor Histocompatibility Antigens
- RNA, Messenger
- APOBEC3B protein, human
- Cytidine Deaminase
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Topics |
- Carcinoma, Hepatocellular
(pathology)
- Cell Line, Tumor
- Cell Movement
- Cytidine Deaminase
(genetics, physiology)
- Hepatectomy
- Humans
- Liver Neoplasms
(pathology)
- Minor Histocompatibility Antigens
- Neoplasm Recurrence, Local
(etiology)
- RNA, Messenger
(analysis)
- Wound Healing
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