Osteopontin-induced brown adipogenesis from white preadipocytes through a PI3K-AKT dependent signaling.

Abstract

Recent studies have shown that OPN (osteopontin) plays critical roles in cell survival, differentiation, bio-mineralization, cancer and cardiovascular remodeling. However, its roles in the differentiation of brown adipocytes and the underlying mechanisms remain unclear. Therefore, the aim of this study was to investigate the roles of OPN in the brown adipogenesis and the underlying mechanisms. It was shown that the OPN successfully induced the differentiation of 3T3-L1 white preadipocytes into the PRDM16(+) (PRD1-BF1-RIZ1 homologous domain containing 16) and UCP-1(+) (uncoupling protein-1) brown adipocytes in a concentration and time-dependent manner. Also, activation of PI3K (phosphatidylinositol 3-kinase)-AKT pathway was required for the OPN-induced brown adipogenesis. The findings suggest OPN plays an important role in promoting the differentiation of the brown adipocytes and might provide a potential novel therapeutic approach for the treatment of obesity and related disorders.

• Authors: Xiao-Juan Zhong, Xiao-Dan Shen, Jian-Bing Wen, Ying Kong, Jia-Jia Chu, Guo-Qiang Yan, Teng Li, Dan Liu, Meng-Qing Wu, Guo-Hua Zeng, Ming He, Qi-Ren Huang
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 459 Issue 3 Pg. 553-9 (Apr 10 2015) ISSN: 1090-2104 [Electronic] United States
• PMID: 25749339 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Integrin alphaVbeta3
• Recombinant Proteins
• Spp1 protein, mouse
• Osteopontin
• Phosphatidylinositol 3-Kinases
• Proto-Oncogene Proteins c-akt

Topics

• 3T3-L1 Cells
• Adipocytes, White (cytology, metabolism)
• Adipogenesis (genetics, physiology)
• Animals
• Cell Differentiation
• Integrin alphaVbeta3 (metabolism)
• Mesenchymal Stem Cells (cytology, metabolism)
• Mice
• Osteopontin (genetics, metabolism)
• Phosphatidylinositol 3-Kinases (metabolism)
• Proto-Oncogene Proteins c-akt (metabolism)
• Recombinant Proteins (genetics, metabolism)
• Signal Transduction