Pratensein ameliorates β-amyloid-induced cognitive impairment in rats via reducing oxidative damage and restoring synapse and BDNF levels.

Abstract

This study was designed to investigate the protective effect of pratensein against cognitive impairment induced by amyloid beta (1-42) (Aβ1-42) in rats. Aβ1-42 peptide was injected bilaterally in the hippocampus of rat. Next, pratensein was administered orally for 3 weeks. Our findings demonstrated that treatment with pratensein ameliorated learning and memory deficits in Aβ1-42 rat model of AD. Pratensein treatment significantly attenuated neuronal degeneration and apoptosis in hippocampus. Moreover, the over-expression in IL-1β and TNF-α as well as the extensive astrogliosis and microgliosis in hippocampus induced by Aβ1-42 were significantly reduced following administration of pratensein. Concomitantly, pratensein treatment significantly suppressed the activation of NF-κB in hippocampus. In addition, pratensein was able to increase the levels of synaptophysin and brain-derived neurotrophic factor (BDNF). These results indicate that pratensein could significantly ameliorate Aβ1-42-induced spatial learning and memory impairment through reducing neuroinflammation via inhibition of glial activation and NF-κB activation, and restoring synapse and BDNF levels, suggesting that administration of pratensein could likely provide a therapeutic approach for AD.

• Authors: Chunhong Liang, Shimei Tan, Quanfang Huang, Jun Lin, Zhongpeng Lu, Xing Lin
• Journal: Neuroscience letters (Neurosci Lett) Vol. 592 Pg. 48-53 (Apr 10 2015) ISSN: 1872-7972 [Electronic] Ireland
• PMID: 25748315 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Amyloid beta-Peptides
• Brain-Derived Neurotrophic Factor
• Interleukin-1beta
• Isoflavones
• NF-kappa B
• Neuroprotective Agents
• Peptide Fragments
• Synaptophysin
• Tumor Necrosis Factor-alpha
• amyloid beta-protein (1-42)
• 4'-methoxy-3',5,7-trihydroxyisoflavone

Topics

• Amyloid beta-Peptides (metabolism, toxicity)
• Animals
• Apoptosis (drug effects)
• Avoidance Learning (drug effects)
• Brain (drug effects, metabolism, pathology)
• Brain-Derived Neurotrophic Factor (metabolism)
• Cognition Disorders (drug therapy, metabolism, psychology)
• Interleukin-1beta (metabolism)
• Isoflavones (pharmacology, therapeutic use)
• Male
• Maze Learning (drug effects)
• NF-kappa B (metabolism)
• Neuroglia (drug effects, metabolism)
• Neurons (drug effects, pathology)
• Neuroprotective Agents (pharmacology, therapeutic use)
• Oxidative Stress
• Peptide Fragments (metabolism, toxicity)
• Rats, Wistar
• Synapses (drug effects, metabolism)
• Synaptophysin (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)