Evaluation of viremia, proviral load and cytokine profile in naturally feline immunodeficiency virus infected cats treated with two different protocols of recombinant feline interferon omega.

Abstract

This study assesses viremia, provirus and blood cytokine profile in naturally FIV-infected cats treated with two distinct protocols of interferon omega (rFeIFN-ω). Samples from FIV-cats previously submitted to two single-arm studies were used: 7/18 received the licensed/subcutaneous protocol (SC) while 11/18 were treated orally (PO). Viremia, provirus and blood mRNA expression of interleukin (IL)-1, IL-4, IL-6, IL-10, IL-12p40, Interferon-γ and Tumor Necrosis Factor-α were monitored by Real-Time qPCR. Concurrent plasma levels of IL-6, IL-12p40 and IL-4 were assessed by ELISA. IL-6 plasma levels decreased in the SC group (p = 0.031). IL-6 mRNA expression (p = 0.037) decreased in the PO group, albeit not sufficiently to change concurrent plasma levels. Neither viremia nor other measured cytokines changed with therapy. Proviral load increased in the SC group (p = 0.031), which can be justified by a clinically irrelevant increase of lymphocyte count. Independently of the protocol, rFeIFN-ω seems to act on innate immunity by reducing pro-inflammatory stimulus.

• Authors: Rodolfo O Leal, Solange Gil, Ana Duarte, David McGahie, Nuno Sepúlveda, Maria M R E Niza, Luís Tavares
• Journal: Research in veterinary science (Res Vet Sci) Vol. 99 Pg. 87-95 (Apr 2015) ISSN: 1532-2661 [Electronic] England
• PMID: 25747956 (Publication Type: Comparative Study, Journal Article)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• Cytokines
• Immunologic Factors
• Interferon Type I
• Interleukin-12 Subunit p40
• Interleukin-6
• RNA, Messenger
• Recombinant Proteins
• interferon omega 1
• Interleukin-4

Topics

• Administration, Oral
• Animals
• Cat Diseases (blood, immunology, virology)
• Cats (virology)
• Cytokines (blood)
• Feline Acquired Immunodeficiency Syndrome (blood, drug therapy, immunology)
• Immunodeficiency Virus, Feline (immunology)
• Immunologic Factors (administration & dosage, pharmacology, therapeutic use)
• Injections, Subcutaneous
• Interferon Type I (administration & dosage, pharmacology, therapeutic use)
• Interleukin-12 Subunit p40 (blood)
• Interleukin-4 (blood)
• Interleukin-6 (blood)
• RNA, Messenger (blood)
• Recombinant Proteins (administration & dosage, pharmacology, therapeutic use)
• Treatment Outcome
• Viral Load (drug effects, immunology)
• Viremia (blood, immunology, veterinary)