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Boldine, a natural aporphine alkaloid, inhibits telomerase at non-toxic concentrations.

Abstract
In a preliminary screening study of natural alkaloids, boldine, an aporphine alkaloid, showed an interesting dose and time dependent anti-proliferative effect in several cancer cell lines. Cytotoxicity of boldine in human fibroblasts was considerably lower than the telomerase positive embryonic kidney HEK293 and breast cancer MCF-7 and MDA-MB-231 cells. Whether boldine can inhibit telomerase was investigated here using a modified quantitative real-time telomere repeat amplification protocol (q-TRAP). This test showed that boldine inhibits telomerase in cells treated with sub-cytotoxic concentrations. Telomerase inhibition occurs via down-regulation of hTERT, the catalytic subunit of the enzyme. Boldine changed the splicing variants of hTERT towards shorter non-functional transcripts as well. A direct interaction of boldine with the enzyme may also be involved, though thermal FRET method did not detect any substantial interaction between boldine and synthetic telomere sequences. This study advocates boldine as a valuable candidate for telomerase-targeted cancer care. This study suggests that derivatives of boldine could be potent anti-cancer drugs.
AuthorsSakineh Kazemi Noureini, Fatemeh Tanavar
JournalChemico-biological interactions (Chem Biol Interact) Vol. 231 Pg. 27-34 (Apr 25 2015) ISSN: 1872-7786 [Electronic] Ireland
PMID25746354 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2015 Elsevier Ireland Ltd. All rights reserved.
Chemical References
  • Antineoplastic Agents
  • Antioxidants
  • Aporphines
  • Protein Isoforms
  • boldine
  • Telomerase
Topics
  • Antineoplastic Agents (pharmacology)
  • Antioxidants (pharmacology)
  • Aporphines (pharmacology)
  • Breast (drug effects, metabolism, pathology)
  • Breast Neoplasms (drug therapy, metabolism, pathology)
  • Cell Line, Tumor
  • Down-Regulation (drug effects)
  • Female
  • HEK293 Cells
  • Humans
  • Protein Isoforms (antagonists & inhibitors, genetics, metabolism)
  • Telomerase (antagonists & inhibitors, genetics, metabolism)

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