Hydroxytyrosol, a polyphenolic compound from extra virgin
olive oil (EVOO) has exhibited an improvement in a model of DSS-induced
colitis. However, other phenolic compounds present such as
hydroxytyrosyl acetate (HTy-Ac) and
3,4-dihydroxyphenylglycol (
DHPG) need to be explored to complete the understanding of the overall effects of EVOO on inflammatory colon mucosa. This study was designed to evaluate the effect of both HTy-Ac and
DHPG dietary supplementation in the inflammatory response associated to
colitis model. Six-week-old mice were randomized in four dietary groups:
sham and control groups received standard diet, and other two groups were fed with HTy-Ac and
DHPG, respectively, at 0.1%. After 30 days, all groups except
sham received 3% DSS in
drinking water for 5 days followed by a regime of 5 days of water. Acute
inflammation was evaluated by Disease Activity Index (DAI), histology and
myeloperoxidase (MPO) activity. Colonic expression of iNOS, COX-2, MAPKs,
NF-kB and FOXP3 were determined by western blotting. Only HTy-Ac-supplemented group showed a significant DAI reduction as well as an improvement of histological damage and MPO. COX-2 and iNOS
protein expression were also significantly reduced. In addition, this dietary group down-regulated JNK phosphorylation and prevented the DSS-induced nuclear translocation level of p65. However, no significant differences were observed in the FOXP3 expression. These results demonstrated, for the first time, that HTy-Ac exerts an antiinflammatory effect on acute
ulcerative colitis. We concluded that HTy-Ac supplement might provide a basis for developing a new dietary strategy for the prevention of
ulcerative colitis.