Oral vaccination of mice with Trichinella spiralis nudix hydrolase DNA vaccine delivered by attenuated Salmonella elicited protective immunity.

Abstract

We have previously reported that Trichinella spiralis Nudix hydrolase (TsNd) bound to intestinal epithelial cells (IECs), and the vaccination of mice with recombinant TsNd protein (rTsNd) produced a partial protective immunity against challenge infection in mice. In this study, the full-length cDNA sequence of TsNd gene was cloned into the eukaryotic expression plasmid pcDNA3.1, and the recombinant TsNd DNA was transformed into attenuated Salmonella typhimurium strain ⊿cyaSL1344. Oral immunization of mice with TsNd/S. typhimurium elicited a significant local mucosal IgA response and a systemic Th1/Th2 immune response. Cytokine profiling also showed a significant increase in the Th1 (IFN-γ, IL-2) and Th2 (IL-4, 10) responses in splenocytes of immunized mice upon stimulation with the rTsNd. The oral immunization of mice with TsNd/S. typhimurium displayed a statistically significant 73.32% reduction in adult worm burden and a 49.5% reduction in muscle larvae after challenge with T. spiralis muscle larvae, compared with PBS control group. Our results demonstrated that TsNd DNA delivered by attenuated live S. typhimurium elicited a local IgA response and a mixed Th1/Th2 immune response, and produced a partial protection against T. spiralis infection in mice.

• Authors: Pei Liu, Zhong Quan Wang, Ruo Dan Liu, Peng Jiang, Shao Rong Long, Li Na Liu, Xin Zhuo Zhang, Xiang Chao Cheng, Chuan Yu, Hui Jun Ren, Jing Cui
• Journal: Experimental parasitology (Exp Parasitol) Vol. 153 Pg. 29-38 (Jun 2015) ISSN: 1090-2449 [Electronic] United States
• PMID: 25733024 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Antibodies, Helminth
• Cytokines
• Vaccines
• Vaccines, DNA
• Pyrophosphatases
• nudix hydrolases

Topics

• Administration, Oral
• Animals
• Antibodies, Helminth (immunology)
• Cytokines (immunology)
• Drug Delivery Systems
• Female
• Genetic Vectors (genetics, immunology)
• Humans
• Immunity, Cellular
• Male
• Mice
• Mice, Inbred BALB C
• Pyrophosphatases (administration & dosage, genetics, immunology)
• Salmonella typhimurium (genetics, immunology)
• Th1 Cells (immunology)
• Th2 Cells (immunology)
• Trichinella spiralis (enzymology, genetics, immunology)
• Trichinellosis (immunology, parasitology, prevention & control)
• Vaccination
• Vaccines (administration & dosage, genetics, immunology)
• Vaccines, DNA (administration & dosage, genetics, immunology)