Abstract | OBJECTIVE: The study aimed to investigate whether intravitreal injection of mesenchymal stem cells (MSCs) cultivated in vitro could increase the number of survived retinal ganglion cells (RGCs) after traumatic optic neuropathy and sought to identify potential mechanisms underlying such growth. METHODS: RESULTS: Quantitative analysis showed that RGCs were significantly attenuated at 3, 7, 14, and 28 days after transplantation in both areas of the retina (peripheral, P7d = 0.0011, P14d < 0.001, P28d < 0.001; central, P3d = 0.0437, P7d = 0.0067, P14d < 0.001, P28d < 0.001). Mean density of RGCs in the MSC transplantation group was significantly higher than that of the PBS control group after 14 days of treatment (P < 0.001). The homogenates BDNF (hBDNF) in the MSC transplantation group was obviously higher than that in the PBS control group at 14 and 28 days (P < 0.05). CONCLUSIONS: The MSCs transplanted into the retina of cats can slow down RGC apoptosis and steadily express BDNF. The MSC-mediated neuroprotection after optic nerve injury may be related to BNDF.
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Authors | Li Junyi, Li Na, Jiang Yan |
Journal | The Journal of craniofacial surgery
(J Craniofac Surg)
Vol. 26
Issue 2
Pg. 548-52
(Mar 2015)
ISSN: 1536-3732 [Electronic] United States |
PMID | 25723663
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Brain-Derived Neurotrophic Factor
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Topics |
- Animals
- Brain-Derived Neurotrophic Factor
(metabolism)
- Cats
- Cell Survival
(physiology)
- Female
- Intravitreal Injections
- Male
- Mesenchymal Stem Cell Transplantation
- Mesenchymal Stem Cells
(metabolism)
- Optic Nerve Injuries
(physiopathology)
- Rats, Sprague-Dawley
- Retinal Ganglion Cells
(physiology)
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