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A novel antioxidant multitarget iron chelator M30 protects hepatocytes against ethanol-induced injury.

Abstract
The multitarget iron chelator, M30, is a novel antioxidant and protective agent against oxidative stress in a spectrum of diseases. However, there is no report regarding its role in liver diseases. Since oxidative stress is one of the major pathological events during the progression of alcoholic liver diseases, the protective effects and mechanisms of M30 on ethanol-induced hepatocyte injury were investigated in this study. Rat hepatocyte line BRL-3A was pretreated with M30 prior to ethanol treatment. Cell death, apoptosis, oxidative stress, and inflammation were examined. Specific antagonists and agonists were applied to determine the involvements of hypoxia inducible factor-1 alpha (HIF-1α) and its upstream adenylate cyclase (AC)/cyclic AMP (cAMP)/protein kinase A (PKA)/HIF-1α/NOD-like receptor 3 (NLRP3) inflammasome pathway. We found that M30 significantly attenuated ethanol-induced cellular death, apoptosis, production of reactive oxygen species (ROS), and secretion of inflammatory cytokines and inhibited activation of the AC/cAMP/PKA/HIF-1α/NLRP3 inflammasome pathway. Inhibition and activation of the AC/cAMP/PKA/HIF-1α pathway mimicked and abolished the effects of M30, respectively. In conclusion, inhibition of the AC/cAMP/PKA/HIF-1α/NLRP3 inflammasome pathway by M30 partially contributes to its attenuation of hepatocyte injury caused by ethanol exposure.
AuthorsJia Xiao, Yi Lv, Bin Lin, George L Tipoe, Moussa B H Youdim, Feiyue Xing, Yingxia Liu
JournalOxidative medicine and cellular longevity (Oxid Med Cell Longev) Vol. 2015 Pg. 607271 ( 2015) ISSN: 1942-0994 [Electronic] United States
PMID25722794 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • 5-(N-methyl-N-propargylaminomethyl)-8-hydroxyquinoline
  • Antioxidants
  • Carrier Proteins
  • Chelating Agents
  • Hydroxyquinolines
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • NLR Family, Pyrin Domain-Containing 3 Protein
  • Nlrp3 protein, rat
  • Reactive Oxygen Species
  • Receptors, Cytoplasmic and Nuclear
  • Ethanol
  • Cyclic AMP
  • Cyclic AMP-Dependent Protein Kinases
  • Adenylyl Cyclases
Topics
  • Adenylyl Cyclases (metabolism)
  • Animals
  • Antioxidants (chemistry, pharmacology)
  • Apoptosis (drug effects)
  • Carrier Proteins
  • Cell Line
  • Chelating Agents (chemistry, pharmacology)
  • Cyclic AMP (metabolism)
  • Cyclic AMP-Dependent Protein Kinases (metabolism)
  • Ethanol (toxicity)
  • Hepatocytes (cytology, drug effects, metabolism)
  • Hydroxyquinolines (chemistry, pharmacology)
  • Hypoxia-Inducible Factor 1, alpha Subunit (metabolism)
  • NLR Family, Pyrin Domain-Containing 3 Protein
  • Oxidative Stress (drug effects)
  • Rats
  • Reactive Oxygen Species (metabolism)
  • Receptors, Cytoplasmic and Nuclear (metabolism)
  • Signal Transduction (drug effects)

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