Abstract | BACKGROUND: AIMS: MATERIALS AND METHODS: Severe hemorrhage was induced by arterial controlled bleeding of 50% of the blood volume (BV) and the hypovolemic state was maintained for 1 h. The animals received volume resuscitation by an infusion of 25% of BV using fresh blood alone or with added nitrite, or fresh blood treated with RRx-001 (140 mg/kg) or RRx-001 (140 mg/kg) with added nitrite. Systemic and microvascular hemodynamics were followed at baseline and at different time points during the entire study. Tissue apoptosis and necrosis were measured 8 h after resuscitation to correlate hemodynamic changes with tissue viability. RESULTS: Compared to resuscitation with blood alone, blood treated with RRx-001 decreased vascular resistance, increased blood flow and functional capillary density immediately after resuscitation and preserved tissue viability. Furthermore, in RRx-001 treated animals, both mean arterial pressure (MAP) and met Hb were maintained within normal levels after resuscitation (MAP >90 mmHg and metHb <2%). The addition of nitrite to RRx-001 did not significantly improve the effects of RRx-001, as it increased methemoglobinemia and lower MAP. CONCLUSION:
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Authors | Chad Brouse, Daniel Ortiz, Yan Su, Bryan Oronsky, Jan Scicinski, Pedro Cabrales |
Journal | Asian journal of transfusion science
(Asian J Transfus Sci)
2015 Jan-Jun
Vol. 9
Issue 1
Pg. 55-60
ISSN: 0973-6247 [Print] India |
PMID | 25722574
(Publication Type: Journal Article)
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