An ethanol root extract of Cynanchum wilfordii containing acetophenones suppresses the expression of VCAM-1 and ICAM-1 in TNF-α-stimulated human aortic smooth muscle cells through the NF-κB pathway.

Abstract

The root of Cynanchum wilfordii (C. wilfordii) contains several biologically active compounds which have been used as traditional medicines in Asia. In the present study, we evaluated the anti-inflammatory effects of an ethanol root extract of C. wilfordii (CWE) on tumor necrosis factor (TNF)-α-stimulated human aortic smooth muscle cells (HASMCs). The inhibitory effects of CWE on vascular cell adhesion molecule (VCAM)-1 expression under an optimum extraction condition were examined. CWE suppressed the expression of VCAM-1 and ICAM-1 and the adhesion of THP-1 monocytes to the TNF-α-stimulated HASMCs. Consistent with the in vitro observations, CWE inhibited the aortic expression of ICAM-1 and VCAM-1 in atherogenic diet-fed mice. CWE also downregulated the expression of nuclear factor-κB (NF-κB p65) and its uclear translocation in the stimulated HASMCs. In order to identify the active components in CWE, we re-extracted CWE using several solvents, and found that the ethyl acetate fraction was the most effective in suppressing the expression of VCAM-1 and ICAM-1. Four major acetophenones were purified from the ethyl acetate fraction, and two components, p-hydroxyacetophenone and cynandione A, potently inhibited the expression of ICAM-1 and VCAM-1 in the stimulated HASMCs. We assessed and determined the amounts of these two active components from CWE, and our results suggested that the root of C. wilfordii and its two bioactive acetophenones may be used for the prevention and treatment of atherosclerosis and vascular inflammatory diseases.

• Authors: Hyun Jung Koo, Eun-Hwa Sohn, Suhkneung Pyo, Han Goo Woo, Dae Won Park, Young-Min Ham, Seon-A Jang, Soo-Yeong Park, Se Chan Kang
• Journal: International journal of molecular medicine (Int J Mol Med) Vol. 35 Issue 4 Pg. 915-24 (Apr 2015) ISSN: 1791-244X [Electronic] Greece
• PMID: 25716870 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acetophenones
• NF-kappa B
• Plant Extracts
• Tumor Necrosis Factor-alpha
• Vascular Cell Adhesion Molecule-1
• Intercellular Adhesion Molecule-1

Topics

• Acetophenones (pharmacology)
• Active Transport, Cell Nucleus
• Animals
• Aorta (cytology, drug effects)
• Cell Adhesion (drug effects)
• Cell Survival (drug effects)
• Cynanchum (chemistry)
• Gene Expression Regulation (drug effects)
• Humans
• Intercellular Adhesion Molecule-1 (genetics, metabolism)
• Male
• Mice
• Monocytes (drug effects, metabolism)
• Myocytes, Smooth Muscle (drug effects, metabolism)
• NF-kappa B (metabolism)
• Plant Extracts (chemistry, pharmacology)
• Plant Roots (chemistry)
• Tumor Necrosis Factor-alpha (pharmacology)
• Vascular Cell Adhesion Molecule-1 (genetics, metabolism)