Influence of nitric oxide synthase or cyclooxygenase inhibitors on cannabinoids activity in streptozotocin-induced neuropathy.

Abstract

BACKGROUND:

Influence of a relatively specific inhibitor cyclooxygenase (COX)-2, celecoxib, a relatively specific inhibitor of neuronal nitric oxide synthase (NOS), 7-Ni, and a relatively selective inhibitor of inducible NOS, L-NIL, on the action of a preferentially selective CB1 cannabinoid receptor agonist, Met-F-AEA and a selective CB2 cannabinoid receptor agonist, AM 1241 was investigated, in a streptozotocin (STZ)-induced neuropathy.

METHODS:

Studies were performed on male Wistar rats. Changes in nociceptive thresholds were determined using mechanical stimuli - the modification of the classic paw withdrawal test described by Randall-Selitto. Diabetes was induced by a single administration of STZ.

RESULTS:

In a diabetic neuropathic pain model, pretreatment with celecoxib, L-NIL and 7-Ni, significantly increased the antihyperalgesic activity of both Met-F-AEA and AM 1241.

CONCLUSIONS:

The results of this study seemed to indicate that the interaction between cannabinoid, COX-2 and NOS(s) systems might exist. Concomitant administration of small doses of CB1 and/or CB2 receptor agonists and COX-2 or NOS inhibitors can be effective in the alleviation of diabetic neuropathic pain.

• Authors: Magdalena Bujalska-Zadrożny, Anna de Cordé, Karolina Pawlik
• Journal: Pharmacological reports : PR (Pharmacol Rep) Vol. 67 Issue 2 Pg. 209-16 (Apr 2015) ISSN: 2299-5684 [Electronic] Switzerland
• PMID: 25712641 (Publication Type: Journal Article)
• Copyright: Copyright © 2014 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

Chemical References

• 2-methylarachidonyl-2'-fluoroethylamide
• AM 1241
• Arachidonic Acids
• Cannabinoids
• Cyclooxygenase Inhibitors
• Indazoles
• N(6)-(1-iminoethyl)lysine
• Streptozocin
• Nitric Oxide Synthase Type I
• Nitric Oxide Synthase Type II
• Celecoxib
• Lysine
• 7-nitroindazole

Topics

• Animals
• Arachidonic Acids (pharmacology, therapeutic use)
• Cannabinoids (pharmacology, therapeutic use)
• Celecoxib (pharmacology, therapeutic use)
• Cyclooxygenase Inhibitors (pharmacology, therapeutic use)
• Diabetic Neuropathies (chemically induced, complications, drug therapy)
• Drug Synergism
• Hyperalgesia (drug therapy)
• Indazoles (pharmacology, therapeutic use)
• Lysine (analogs & derivatives, pharmacology, therapeutic use)
• Male
• Neuralgia (complications, drug therapy)
• Nitric Oxide Synthase Type I (antagonists & inhibitors)
• Nitric Oxide Synthase Type II (antagonists & inhibitors)
• Pain Measurement (drug effects)
• Rats
• Rats, Wistar
• Streptozocin