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Carcinostatic effects of platinum nanocolloid combined with gamma irradiation on human esophageal squamous cell carcinoma.

AbstractAIMS:
To explore the carcinostatic effects of platinum nanocolloid (Pt-nc) combined with gamma rays on human esophageal squamous cell carcinoma (ESCC).
MAIN METHODS:
ESCC-derived KYSE-70 cells were treated with various concentrations of Pt-nc and/or gamma irradiation, and subsequently cultured in phenol red free DMEM with 10% FBS for 48 h. The proliferative status of the KYSE-70 cells was evaluated using trypan blue dye exclusion and WST-8 assays. Cellular and nucleic morphological aspects were evaluated using crystal violet and Hoechst 33342 stainings, respectively. Radiosensitivity was quantified by a cell viability assay, and the activated form of caspase-3, a characteristic apoptosis-related protein, was detected by Western blotting.
KEY FINDINGS:
Although single treatment with either Pt-nc or gamma irradiation could slightly inhibit the growth of the KYSE-70 cells, their combination exerted remarkable carcinostatic effects in a manner dependent on either Pt-nc concentrations or gamma ray doses, compared with the effect of each treatment alone (p<0.05). By fluorescence micrographic observation, the KYSE-70 cells that were treated with Pt-nc and subsequently irradiated with gamma rays, were shown to undergo distinct apoptotic morphological changes. The carcinostatic effect of gamma rays at 7 Gy without Pt-nc was approximately equal to that when 3-Gy irradiation was combined with 100 ppm Pt-nc or that 5-Gy irradiation was combined with 50 ppm Pt-nc.
SIGNIFICANCE:
Pt-nc in combination with gamma rays may exert a cooperative effect through platinum- or gamma ray-induced apoptosis resulting in the inhibition of growth of cancer cells, while concurrently enabling the lowering of the radiative dose.
AuthorsQiang Li, Yoshiharu Tanaka, Yasukazu Saitoh, Hiroshi Tanaka, Nobuhiko Miwa
JournalLife sciences (Life Sci) Vol. 127 Pg. 106-14 (Apr 15 2015) ISSN: 1879-0631 [Electronic] Netherlands
PMID25711430 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2015 Elsevier Inc. All rights reserved.
Chemical References
  • Antineoplastic Agents
  • Colloids
  • Platinum Compounds
  • polyvinylpolypyrrolidone
  • Caspase 3
  • Povidone
Topics
  • Antineoplastic Agents (pharmacology)
  • Apoptosis (drug effects, radiation effects)
  • Carcinoma, Squamous Cell (drug therapy, radiotherapy)
  • Caspase 3 (drug effects, radiation effects)
  • Cell Line, Tumor
  • Cell Proliferation (drug effects, radiation effects)
  • Cell Survival (drug effects, radiation effects)
  • Colloids
  • Enzyme Activation (drug effects, radiation effects)
  • Esophageal Neoplasms (drug therapy, radiotherapy)
  • Esophageal Squamous Cell Carcinoma
  • Gamma Rays
  • Humans
  • Nanostructures
  • Platinum Compounds (pharmacology)
  • Povidone (analogs & derivatives, chemistry)

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