Abstract | AIMS: MAIN METHODS: ESCC-derived KYSE-70 cells were treated with various concentrations of Pt-nc and/or gamma irradiation, and subsequently cultured in phenol red free DMEM with 10% FBS for 48 h. The proliferative status of the KYSE-70 cells was evaluated using trypan blue dye exclusion and WST-8 assays. Cellular and nucleic morphological aspects were evaluated using crystal violet and Hoechst 33342 stainings, respectively. Radiosensitivity was quantified by a cell viability assay, and the activated form of caspase-3, a characteristic apoptosis-related protein, was detected by Western blotting. KEY FINDINGS: Although single treatment with either Pt-nc or gamma irradiation could slightly inhibit the growth of the KYSE-70 cells, their combination exerted remarkable carcinostatic effects in a manner dependent on either Pt-nc concentrations or gamma ray doses, compared with the effect of each treatment alone (p<0.05). By fluorescence micrographic observation, the KYSE-70 cells that were treated with Pt-nc and subsequently irradiated with gamma rays, were shown to undergo distinct apoptotic morphological changes. The carcinostatic effect of gamma rays at 7 Gy without Pt-nc was approximately equal to that when 3-Gy irradiation was combined with 100 ppm Pt-nc or that 5-Gy irradiation was combined with 50 ppm Pt-nc. SIGNIFICANCE: Pt-nc in combination with gamma rays may exert a cooperative effect through platinum- or gamma ray-induced apoptosis resulting in the inhibition of growth of cancer cells, while concurrently enabling the lowering of the radiative dose.
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Authors | Qiang Li, Yoshiharu Tanaka, Yasukazu Saitoh, Hiroshi Tanaka, Nobuhiko Miwa |
Journal | Life sciences
(Life Sci)
Vol. 127
Pg. 106-14
(Apr 15 2015)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 25711430
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Inc. All rights reserved. |
Chemical References |
- Antineoplastic Agents
- Colloids
- Platinum Compounds
- polyvinylpolypyrrolidone
- Caspase 3
- Povidone
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Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects, radiation effects)
- Carcinoma, Squamous Cell
(drug therapy, radiotherapy)
- Caspase 3
(drug effects, radiation effects)
- Cell Line, Tumor
- Cell Proliferation
(drug effects, radiation effects)
- Cell Survival
(drug effects, radiation effects)
- Colloids
- Enzyme Activation
(drug effects, radiation effects)
- Esophageal Neoplasms
(drug therapy, radiotherapy)
- Esophageal Squamous Cell Carcinoma
- Gamma Rays
- Humans
- Nanostructures
- Platinum Compounds
(pharmacology)
- Povidone
(analogs & derivatives, chemistry)
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