Abstract |
Highly electronegative low-density lipoprotein ( LDL) L5 induces endothelial cell (EC) apoptosis, which leads to the development of atherosclerosis. We examined the effects of sesamol (1), a natural organic component of sesame oil, on plasma L5 levels and atherosclerosis development in a rodent model and on the L5-induced apoptosis of ECs. Syrian hamsters, which have an LDL profile similar to that of humans, were fed a normal chow diet (control), a high-fat diet (HFD), or a HFD supplemented with the administration of 50 or 100 mg/kg of 1 via oral gavage (HFD+1) for 16 weeks (n = 8 per group). Hamsters in the HFD+1 groups had reduced plasma L5 levels when compared with the HFD group. Oil Red O staining showed that atherosclerotic lesion size was markedly reduced in the aortic arch of hamsters in the HFD+1 groups when compared with that in the HFD group. In human aortic ECs, 0.3-3 μM 1 blocked L5-induced apoptosis in a dose-dependent manner. Further mechanistic studies showed that 1 inhibited the L5-induced lectin-like oxidized LDL receptor-1 (LOX-1)-dependent phosphorylation of p38 MAPK and activation of caspase-3 and increased phosphorylation of eNOS and Akt. Our findings suggest that sesamol (1) protects against atherosclerosis by reducing L5-induced atherogenicity.
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Authors | Wei-Yu Chen, Fang-Yu Chen, An-Sheng Lee, Kuan-Hsiang Ting, Chia-Ming Chang, Jing-Fang Hsu, Wei-Shine Lee, Joen-Rong Sheu, Chu-Huang Chen, Ming-Yi Shen |
Journal | Journal of natural products
(J Nat Prod)
Vol. 78
Issue 2
Pg. 225-33
(Feb 27 2015)
ISSN: 1520-6025 [Electronic] United States |
PMID | 25692815
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Benzodioxoles
- Lipoproteins, LDL
- OLR1 protein, human
- Phenols
- Scavenger Receptors, Class E
- oxidized low density lipoprotein
- sesamol
- Caspase 3
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Topics |
- Animals
- Apoptosis
(drug effects)
- Atherosclerosis
(drug therapy)
- Benzodioxoles
(blood, chemistry, pharmacology)
- Blotting, Western
- Caspase 3
- Cricetinae
- Dose-Response Relationship, Drug
- Endothelial Cells
(drug effects)
- Humans
- In Vitro Techniques
- Lipoproteins, LDL
(analysis, blood, drug effects)
- Male
- Molecular Structure
- Phenols
(blood, chemistry, pharmacology)
- Scavenger Receptors, Class E
(blood, drug effects)
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