Dendritic cells induce specific cytotoxic T lymphocytes against prostate cancer TRAMP-C2 cells loaded with freeze- thaw antigen and PEP-3 peptide.

Abstract

Prostate cancer is the most common cancer in men. In this study, we investigated immune responses of cytotoxic T lymphocytes (CTLs) against TRAMP-C2 prostate cancer cells after activation by dendritic cells (DCs) loaded with TRAMP-C2 freeze-thaw antigen and/or PEP-3 peptide in vitro. Bone marrow-derived DC from the bone marrow of the C57BL/6 were induced to mature by using the cytokine of rhGM-CSF and rhIL-4, and loaded with either the freeze-thaw antigen or PEP-3 peptide or both of them. Maturation of DCs was detected by flow cytometry. The killing efficiency of the CTLs on TRAMP-C2 cells were detected by flow cytometry, CCK8, colony formation, transwell migration, and wound-healing assay. The levels of the IFN-γ, TNF-β and IL-12 were measured by enzyme-linked immunosorbent assay (ELISA). Compared with the unloaded DCs, the loaded DCs had significantly increased expression of several phenotypes related to DC maturation. CTLs activated by DCs loaded with freeze-thaw antigen and PEP-3 peptide had more evident cytotoxicity against TRAMP-C2 cells in vitro. The secretion levels of IFN-γ, TNF-β and IL-12, secreted by DCs loaded with antigen and PEP-3 and interaction with T cells, were higher than in the other groups. Our results suggest that the CTLs activated by DCs loaded with TRAMP-C2 freeze-thaw antigen and PEP-3 peptide exert a remarkable killing efficiency against TRAMP-C2 cells in vitro.

• Authors: Xiao-Qi Liu, Rong Jiang, Si-Qi Li, Jing Wang, Fa-Ping Yi
• Journal: Asian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 16 Issue 2 Pg. 571-8 ( 2015) ISSN: 2476-762X [Electronic] Thailand
• PMID: 25684489 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Cytokines
• Pep-3 peptide
• Peptides

Topics

• Animals
• Antigens, Neoplasm (immunology, metabolism)
• Apoptosis
• Blotting, Western
• Cell Movement
• Cell Proliferation
• Cells, Cultured
• Cytokines (metabolism)
• Dendritic Cells (immunology, metabolism, pathology)
• Flow Cytometry
• Freezing
• Humans
• Male
• Mice
• Mice, Inbred C57BL
• Peptides (immunology, metabolism)
• Prostatic Neoplasms (immunology, metabolism, pathology)
• T-Lymphocytes, Cytotoxic (immunology, metabolism, pathology)
• Wound Healing
• Xenograft Model Antitumor Assays