Abstract | OBJECTIVES: Erythrina lysistemon was found to improve lipid profile in ovariectomized rats. Alpinumisoflavone (AIF) and abyssinone V 4'-methylether ( AME) derived from this plant induced analogous effects on lipid profile and decreased atherogenic risks. To highlight the molecular mechanism of action of these natural products, we evaluated their effects on the expression of some estrogen-sensitive genes associated with cholesterol synthesis (Esr1 and Apoa1) and cholesterol clearance (Ldlr, Scarb1 and Cyp7a1). METHODS: Ovariectomized rats were subcutaneously treated for three consecutive days with either compound at the daily dose of 0.1, 1 and 10 mg/kg body weight (BW). Animals were sacrificed thereafter and their liver was collected. The mRNA of genes of interest was analysed by quantitative real-time polymerase chain reaction. KEY FINDINGS: CONCLUSION: AIF and AME atheroprotective effects may result from their ability to upregulate mechanisms promoting HDL-cholesterol and bile acid formation.
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Authors | Marie A Mvondo, Dieudonné Njamen, Georg Kretzschmar, Manuela Imma Bader, Stephen Tanee Fomum, Jean Wandji, Günter Vollmer |
Journal | The Journal of pharmacy and pharmacology
(J Pharm Pharmacol)
Vol. 67
Issue 7
Pg. 990-6
(Jul 2015)
ISSN: 2042-7158 [Electronic] England |
PMID | 25683903
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2015 Royal Pharmaceutical Society. |
Chemical References |
- Apolipoprotein A-I
- Cholesterol, HDL
- Cholesterol, LDL
- Estrogen Receptor alpha
- Estrogens
- Flavonoids
- Isoflavones
- Plant Extracts
- RNA, Messenger
- Receptors, LDL
- Scarb1 protein, rat
- Scavenger Receptors, Class B
- alpinumisoflavone
- abyssinone V
- Cholesterol
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Topics |
- Animals
- Apolipoprotein A-I
(metabolism)
- Cholesterol
(metabolism)
- Cholesterol, HDL
(metabolism)
- Cholesterol, LDL
(metabolism)
- Down-Regulation
(drug effects)
- Erythrina
(chemistry)
- Estrogen Receptor alpha
(metabolism)
- Estrogens
(metabolism)
- Fabaceae
(chemistry)
- Female
- Flavonoids
(chemistry, pharmacology)
- Gallstones
(prevention & control)
- Isoflavones
(chemistry, pharmacology)
- Ovariectomy
(methods)
- Plant Extracts
(chemistry, pharmacology)
- RNA, Messenger
(drug effects)
- Rats
- Rats, Wistar
- Receptors, LDL
(metabolism)
- Scavenger Receptors, Class B
(metabolism)
- Up-Regulation
(drug effects)
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