Abstract | OBJECTIVES: Damage and detachment of podocytes and loss into the urine have been implicated in the progression of kidney diseases. The purpose of this study was to investigate the potential role of urine cytology based on SurePath(™) combined with immunoenzyme staining using Wilms' tumour 1 (WT1) antibody as a podocyte marker in the discrimination of normality and non-renal urinary tract disease from kidney disease. METHODS: Sixty-six patients with kidney disease, 45 patients with lower urinary tract disease and 30 healthy volunteers were examined. Urine cytology slides were prepared using the SurePath method and immunoenzyme stained with WT1 antibody, and the number of WT1-positive cells was counted. RESULTS: CONCLUSIONS: The results suggest that WT1 immunoenzyme staining of urine cytology can be used to detect some types of kidney disease.
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Authors | H Ohsaki, T Sofue, K Kawakami, Y Nishijima, T Hara, T Matsunaga, Y Kushida, R Haba, Y Shigematsu, S Irino, Y Norimatsu |
Journal | Cytopathology : official journal of the British Society for Clinical Cytology
(Cytopathology)
Vol. 27
Issue 1
Pg. 43-9
(Feb 2016)
ISSN: 1365-2303 [Electronic] England |
PMID | 25683486
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2015 John Wiley & Sons Ltd. |
Chemical References |
- Antibodies
- Biomarkers
- WT1 Proteins
- WT1 protein, human
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Antibodies
(immunology)
- Biomarkers
(analysis)
- Disease Progression
- Female
- Humans
- Immunoenzyme Techniques
- Kidney Diseases
(diagnosis, urine)
- Male
- Middle Aged
- Podocytes
(chemistry)
- Prostatic Hyperplasia
(diagnosis, urine)
- Staining and Labeling
(methods)
- Urinary Calculi
(diagnosis, urine)
- Urinary Tract Infections
(diagnosis, urine)
- Urine
(cytology)
- WT1 Proteins
(analysis, immunology)
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