Induction of apoptosis by ethanolic extract of mango peel and comparative analysis of the chemical constitutes of mango peel and flesh.

Abstract

The underlying mechanisms of the anticancer activity of the ethanolic extract of mango peel (EEMP) and its constituents were investigated. EEMP induced death of human cervical carcinoma HeLa cells through apoptosis, as evidenced by the increased cell population in the sub-G1 phase and the appearance of fragmented nuclei. Treatment of the cells with EEMP also downregulated anti-apoptotic Bcl-2 expression, resulting in the proteolytic activation of caspase-3, 7, 8, and 9 and the degradation of poly (ADP-ribose) polymerase (PARP) protein. The major components of mango peel were identified by liquid chromatography-electrospray ionisation tandem mass spectrometry and gas chromatography-mass spectrometry. Our data suggest that EEMP is an excellent source of quercetin 3-O-galactoside, mangiferin gallate, isomangiferin gallate, quercetin-3-O-arabinopyranoside, and mangiferin along with unsaturated fatty acids oleic acid, linoleic acid, and ethyl linoleate, which may help to prevent cervical cancer and may be a useful agent for the treatment of some other malignancies.

• Authors: Hyeonji Kim, Hana Kim, Ashik Mosaddik, Rajendra Gyawali, Kwang Seok Ahn, Somi Kim Cho
• Journal: Food chemistry (Food Chem) Vol. 133 Issue 2 Pg. 416-22 (Jul 15 2012) ISSN: 1873-7072 [Electronic] England
• PMID: 25683414 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ltd. All rights reserved.

Chemical References

• Antineoplastic Agents, Phytogenic
• Plant Extracts
• Ethanol

Topics

• Antineoplastic Agents, Phytogenic (chemistry, pharmacology)
• Apoptosis (drug effects)
• Ethanol (chemistry)
• Female
• Gas Chromatography-Mass Spectrometry
• HeLa Cells
• Humans
• Mangifera (chemistry)
• Plant Extracts (chemistry, pharmacology)
• Spectrometry, Mass, Electrospray Ionization
• Uterine Cervical Neoplasms (drug therapy, pathology)